Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle.
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Msed_0638 encodes the catalytic MCM-alpha subunit, and the B12-binding MCM-beta subunit is encoded remotely by Msed_2055.
"the gene encoding MCE (Msed_0639) is adjacent to that encoding the catalytic subunit of MCM-α (Msed_0638), while the gene for the coenzyme B(12)-binding subunit of MCM (MCM-β) is located remotely (Msed_2055)"
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Msed_0638, Msed_2055 and Msed_0639 are transcriptionally induced under autotrophic growth.
"The expression of all three genes was significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO(2) fixation."
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Active MCM requires coexpression of both subunits and forms an alpha2beta2 heterotetramer.
"soluble, active MCM was produced only if MCM-α and MCM-β were coexpressed"
Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota.
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A genomic assignment of the M. sedula MCM subunits, with the large subunit Msed_0638 and the small B12-binding-domain subunit Msed_2055, which overlaps the ArgK-like gene Msed_2056.
"M. sedula as well as the other autotrophic members of the Sulfolobales clearly encode MCMs in their genome (e.g., small subunit Msed_2055 and large subunit Msed_0638)."
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
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Defines the 3HP/4HB cycle in M. sedula, in which succinyl-CoA is formed from acetyl-CoA and two bicarbonate.
"In this system, one acetyl-CoA and two bicarbonate molecules were reductively converted via 3-hydroxypropionate to succinyl-CoA."
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO(2) fixation cycle in extremely thermoacidophilic archaea.
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The kinetic model of the cycle uses the MCE and MCM characterization of Han et al. 2012.
"characterization of MCE and MCM was reported previously (Han et al., 2012)"
UniProt record A4YEG1 (A4YEG1_METS5)