Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:11514575

Molecular cloning and expression of a human chondroitin synthase.

Human recombinant CHSY1 catalyzes both chondroitin-chain sugar-transfer reactions; sustained polymerization was not established with the isolated construct.

PMID:12716890

Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.

Coexpression with CHPF supports human CHSY1-dependent chondroitin polymerization and is distinct from mixing separately expressed proteins.

PMID:19946888

Defining the membrane proteome of NK cells.

YTS membrane-fraction proteomics identifies many integral and associated proteins; the specific CHSY1 peptide evidence has not been independently inspected.

PMID:21129727

Loss of CHSY1, a secreted FRINGE enzyme, causes syndromic brachydactyly in humans via increased NOTCH signaling.

Human cells support an extracellular CHSY1 pool and downstream skeletal/NOTCH consequences of loss; these do not identify a separate NOTCH-directed glycosyltransferase reaction.

Reactome:R-HSA-1971487

Chondroitin polymerase complex transfers GalNAc to CS chain

The pathway models a Golgi polymerase complex performing one of the alternating chondroitin-chain sugar additions.

Reactome:R-HSA-1971491

Chondroitin polymerase complex transfers GlcA to CS chain

The pathway models a Golgi polymerase complex performing one of the alternating chondroitin-chain sugar additions.

Reactome:R-HSA-3595176

Defective CHSY1 does not transfer GalNAc to chondroitin

The disease model represents failure of a normal CHSY1 sugar-transfer step and retains the Golgi target entity.

Reactome:R-HSA-3595178

Defective CHSY1 does not transfer GlcA to chondroitin

The disease model represents failure of a normal CHSY1 sugar-transfer step and retains the Golgi target entity.

PMID:41298522

Structural basis for human chondroitin sulfate chain polymerization.

Purified CHSY1–CHPF or CHSY1–CHPF2 complexes extend glycan acceptors, and full-length CHSY1 catalytic mutants fail to restore cellular chondroitin production.

PMID:42204168

Structural basis of chondroitin sulfate backbone polymer synthesis.

Human CHSY1 domain mutants separate GlcA transfer from GalNAc transfer, with product MS supporting the two reactions in polymerase complexes.

PMID:14975935

Glycosaminoglycan polymerization may enable osmotically inactive Na+ storage in the skin.

Dietary salt loading is associated with increased rat skin Chsy1 transcript abundance, supporting a peripheral expression-response annotation.

file:human/CHSY1/CHSY1-source-evidence.json

CHSY1 source and assay scope index