file:PSEPK/nicF/nicF-uniprot.txt

UniProtKB reviewed entry for nicF

Curated reaction annotation is tied to the original KT2440 primary study.

"Reaction=maleamate + H2O = maleate + NH4(+);"

Deciphering the genetic determinants for aerobic nicotinic acid degradation: the nic cluster from Pseudomonas putida KT2440.

Recombinant NicF extracts show maleamate amidohydrolase activity; conserved D31/K121/C154 support a proposed catalytic mechanism, not a target mutational assay.

PMID:22530095

Genomic analysis of Pseudomonas putida: genes in a genome island are crucial for nicotine degradation.

Purified S16 Nfo, Ami and Iso execute the same downstream reactions but do not establish a complete nicotinate route. S16 resting cells failed to degrade nicotinate in the reported test.

"Attempts to degrade nicotinic acid by resting cells of strain S16 did not result in any degradation after 24 h of incubation"

file:PSEPK/nicF/nicF-deep-research-falcon.md

Falcon deep research report for nicF