recG (PA5345, Q9HTL3) review notes

Literature

recG is co-transcribed with oxyR from a single promoter; a recG::Gm mutant (not an in-frame oxyR deletion) is hypersensitive to UV PMID:10913087, partially complemented in trans PMID:10913087. The recG mutant was also less resistant to H2O2 and cumene hydroperoxide PMID:10913087.
Transposon mutants in ruvB and recG are chromate-hypersensitive, complemented by the wild-type genes, and also sensitive to tellurite, selenite and mitomycin C PMID:16105671.
Yeom et al. report that purified RecG binds palindromic promoters of OxyR-controlled genes and, with ATP and SSB, generates linear DNA that OxyR then binds PMID:22621928. The abstract covers P. aeruginosa, P. putida and E. coli and does not say which organism's RecG was purified; full text not retrievable (PMC record returned no body text).
UniRule (RU363016): RecG catalyses Holliday-junction branch migration and fork regression, with 3'-5' helicase polarity. Domain content: RecG wedge, DEAD/DEAH helicase and helicase C-terminal domains (IPR004609).

Search: PubMed metadata for all GOA-cited PMIDs; PMID:10913087 full text cached and read for recG passages; PMID:16105671 and PMID:22621928 abstract only. NCBI E-utilities and Europe PMC search were unavailable (HTTP 500/503) during this session.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002773745	GO:0003678 DNA helicase activity	4	79 (70 taxa)	GO:0003678 and GO:0009378, IDA, PMID:22621928	UNVERIFIABLE	The abstract describes purified RecG unwinding palindromic promoter DNA in an ATP-dependent way, which fits helicase activity, but does not state whether the purified protein was the P. aeruginosa or P. putida enzyme. The term is certainly right for RecG; no defect filed.	E. coli RecG (P24230; IDA PMID:8428576, EXP 3'-5' helicase), M. tuberculosis RecG (P9WMQ7), B. subtilis RecG (O34942)	Yes
PTN002773745	GO:0006281 DNA repair	2	79 (70 taxa)	GO:0006281, IMP, PMID:10913087 and PMID:16105671	SOUND	Full text (PMID:10913087) shows UV hypersensitivity of a recG::Gm mutant with trans-complementation; PMID:16105671 abstract shows chromate and mitomycin C sensitivity with cosmid complementation. RecG is itself a DNA repair helicase, so this is participation, not an indirect phenotype.	Arabidopsis RECG1 (AT2G01440/F4INA9; TAIR IMP DNA repair, PMID:26462909)	Yes

Both IBDs suit a RecG-orthologue clade (bacterial RecG plus plant organellar RECG1). No GO_CENTRAL defect.

Non-seed PseudoCAP rows: cellular response to UV, oxidative stress, chromate and selenite (IMP) are mutant sensitivities that follow from the DNA repair role; kept as non-core.