Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Enigma, a mitochondrial protein affecting lifespan and oxidative stress response in Drosophila.
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Egm (= CG9006) encodes a 639-aa mitochondrial protein with homology to acyl-CoA dehydrogenases; it is an essential gene (null larvae die at third instar) and localizes to mitochondria.
"Egm encodes the CG9006 gene, which corresponds to a 639-aa protein with homology to ACADs, the enzymes that catalyze the first of four reactions that constitute one cycle of the β-oxidation pathway"
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Egm mutants have reduced triglyceride stores and increased paraquat/oxidative-stress resistance with a female-specific lifespan extension; the beta-oxidation involvement is described as indirect.
"indirect evidence suggests that this protein may be involved in β-oxidation"
Proteomics reveals novel Drosophila seminal fluid proteins transferred at mating.
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Egm was detected by mass spectrometry among transferred male seminal fluid proteins, in the lipid-metabolism functional category. This is a proteomic co-occurrence, not a demonstrated insemination function.
"proteins involved in lipid metabolism"
Using natural variation in Drosophila to discover previously unknown endoplasmic reticulum stress genes.
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Egm was one of six lipid-metabolism-related association candidates for tunicamycin-induced ER-stress survival in the DGRP, suggesting a putative (likely indirect) modifier role in the ER stress response.
"with known or putative roles in lipid metabolism"
Proteomic mapping in live Drosophila tissues using an engineered ascorbate peroxidase.
Dissecting the concordant and disparate roles of NDUFAF3 and NDUFAF4 in mitochondrial complex I biogenesis.
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The Drosophila ortholog of ACAD9 (dACAD9/CG9006/Egm) is a core member of the fly MCIA complex; it regulates biogenesis of the PP-b (ND2) sub-module of complex I, and its knockdown destabilizes the partner MCIA factors dNDUFAF1 and dECSIT in assembly intermediates.
"RNAi-mediated knockdown of dACAD9 resulted in a reduction in the amount of dNDUFAF1 and dECSIT that accumulates in AIs in ACAD9-kd samples"
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The MCIA complex in Drosophila comprises the orthologs of ACAD9, NDUFAF1, ECSIT, TMEM126B and TMEM186, all of which associate with CI subcomplexes/holoenzyme.
"all 5 components of the MCIA complex were associated with subcomplexes in FAF4-kd and FAF3-kd samples"
Analysis of dominant enhancers and suppressors of activated Notch in Drosophila.
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Large-scale second-site screen for dominant modifiers of an activated-Notch rough-eye phenotype; basis for the IGI compound-eye-morphogenesis annotation (Egm as a genetic modifier of eye development).
"a second-site mutagenesis screen was performed to isolate enhancers and suppressors of the eye phenotype caused by expression of these activated Notch molecules"
Human acyl-CoA dehydrogenase-9 plays a novel role in the mitochondrial beta-oxidation of unsaturated fatty acids.
Complex I assembly function and fatty acid oxidation enzyme activity of ACAD9 both contribute to disease severity in ACAD9 deficiency.
Egm full-gene evidence and substrate-scope re-review notes