Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
UniProt entry for archaeal amoA from Nitrosopumilus maritimus
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AmoA is ammonia monooxygenase subunit A
"Ammonia monooxygenase subunit A"
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AmoA is an integral membrane protein with multiple transmembrane domains
"GO:0016020; C:membrane; IEA:UniProtKB-KW"
Ammonia-oxidizing archaea and nitrite-oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system
Deep research report on amoA/D9J262 (Falcon/Edison Scientific Literature)
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Archaeal AMO in Nitrosopumilus maritimus has unexpected complexity (Hodgskiss 2023) - it is not a 3-subunit AmoABC complex but includes the AOA-specific subunits AmoX, AmoY, and AmoZ; AmoA functions as one component of this multi-subunit AOA-specific holoenzyme.
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N. maritimus AMO has extraordinarily high substrate affinity (Km ~133 nM total ammonium, doubling time ~26 h, oxidation activity ~52 mmol ammonium per mg protein per h) and uses NH3 (not NH4+) as the physiological substrate; archaeal AMO lacks the bacterial HAO and many cytochrome c proteins, so downstream electron transfer relies on a copper-centric redox network (multicopper oxidases and blue copper proteins).
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Recent in situ work (Rasmussen 2024) shows amoA is one of the most highly transcribed genes during AOA blooms, with nitrification rates up to 1797 nM/day in coastal sediment, validating AmoA as the rate-limiting catalytic subunit of nitrogen-cycle relevance at ecosystem scale.