Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Dorz1, a novel gene expressed in differentiating cerebellar granule neurons, is down-regulated in Zic1-deficient mouse.
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Mouse Dorz1 (Abhd14a) mRNA peaks at E17.5 in differentiating cerebellar granule neuron precursors and is positively regulated by Zic1.
"These results suggest that Dorz1 expression is positively regulated by Zic1 during cerebellar development."
Identification of sequence determinants for the ABHD14 enzymes.
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As of 2023, ABHD14A lacked functional annotation, in contrast to its mammalian lysine deacetylase paralog ABHD14B.
"While ABHD14A still lacks any functional annotation to date, we"
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High sequence similarity causes automated databases to conflate ABHD14A and ABHD14B as the same enzyme across organisms.
"Given their high sequence similarity, automated databases often wrongly assign ABHD14A and ABHD14B as the same enzyme, and therefore, annotating functions to them in various organisms has been problematic."
Biochemical Characterization of ABHD14A, an Outlying Member of the Metabolic Serine Hydrolase Family.
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An engineered soluble N-terminally truncated human ABHD14A variant turns over short-chain p-nitrophenyl esters with CoA-dependent enhancement in vitro, and full-length human ABHD14A-GFP localizes specifically to the Golgi apparatus in HEK293T cells, while physiological substrates remain to be identified.
"Together, these findings establish the enzymatic activity, mechanistic features, and subcellular localization of ABHD14A while providing essential biochemical tools that can now enable the systematic discovery of its physiological substrates and regulatory contexts."
UniProtKB record for human ABHD14A (Q9BUJ0)
Affinage mechanistic annotation for ABHD14A (human)