Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
De novo genes with an lncRNA origin encode unique human brain developmental functionality.
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The protein studied (localization, knockout, over-expression, mouse knock-in) is the 107-amino-acid hominoid-specific ORF of ENSG00000205704, not the 68-amino-acid upstream ORF that UniProt A0A590UK83 now represents.
"which encodes a putative protein of 107 amino acids located in both cytoplasm and nucleus"
Evolution of a Human-Specific De Novo Open Reading Frame and Its Linked Transcriptional Silencer.
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The 68-aa ORF is ultra-conserved to cartilaginous fish and is the ORF translated in somatic brain tissue; the 107-aa ORF arose de novo in primates.
"In somatic brain tissues, only the 68 aa ORF is translated and not the 107 aa ORF [3]; thus, there is no translational readthrough."
Diverse Processes Drive the Origination and Maturation of an Array of Enhancers and Silencers During a Vast Evolutionary Timescale of a Bicistronic Gene.
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SMIM45 encodes an ancient 68-aa microprotein and a de novo 107-aa protein.
"SMIM45 is also a bicistronic gene, encoding both an ancient 68 aa microprotein and a human-specific, de novo 107 aa protein [7]."
-A novel peptide SMIM45-107aa promotes HCC progression via MTDH pathways and its anticancer peptide derivative.
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The 107-aa product (SMIM45-107aa) binds and stabilises MTDH in hepatocellular carcinoma cells and is cytoplasmic there.
"Immunofluorescent staining further demonstrated SMIM45-107aa and MTDH was co-located in the cytoplasm of HCC cells"
SMIM45 ORF check (UniProt sequence versions mapped to Ensembl transcripts)