GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

Manual orthology transfer is the source of the neuronal process annotations; assessed against the actual mouse donor and complex-level primary experiments.

GO_REF:0000033

Annotation inferences using phylogenetic trees

PAINT supports conserved endosomal transport and BLOC-1 membership.

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Subcellular-location vocabulary mapping provides the lysosomal membrane assertion.

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

Ensembl Compara transfers mouse localization and neuronal functions.

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links

Logical inference derives axon cytoplasm from the neuronal transport assertion.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined electronic annotations support mitochondrial/cytosolic locations and BLOC-1 membership.

PMID:15102850

Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1).

Endogenous human/mouse co-immunoprecipitation and fractionation identify BLOS1, BLOS2, BLOS3 and SNAPIN as BLOC-1 subunits; a binary-interaction network is reported.

PMID:22203680

Assembly and architecture of biogenesis of lysosome-related organelles complex-1 (BLOC-1).

Reconstituted BLOC-1 contains one copy of eight subunits, with a stable pallidin-Cappuccino-BLOS1 subcomplex supporting a structural assembly role.

PMID:22309213

Identification of a molecular component of the mitochondrial acetyltransferase programme: a novel role for GCN5L1.

GCN5L1 has cytosolic and mitochondrial pools, associates with respiratory-chain targets, and supports acetylation that opposes SIRT3-associated respiratory effects.

PMID:23580065

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma naïve to therapy.

Human tear proteomics is the source of the extracellular HDA annotation, but the BLOC1S1 protein-level identification remains unverified here.

PMID:25416956

A proteome-scale map of the human interactome network.

Human binary-interactome mapping supplies one BLOS1-pallidin interaction assertion.

PMID:25898167

BORC, a multisubunit complex that regulates lysosome positioning.

BORC is distinct from BLOC-1; BLOS1 depletion destabilizes both assemblies and disrupts Arl8-dependent lysosome positioning in human HeLa cells.

PMID:31314175

Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.

BORC perturbations affect late endosomal/lysosomal size through PIKfyve-dependent mechanisms, with differing effects among tested subunits.

PMID:32296183

A reference map of the human binary protein interactome.

A human binary-interactome map supplies eight BLOS1 partner assertions, including two partner isoform identifiers.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Affinity-purification networks supply BLOC-1 partner associations.

PMID:38281616

GCN5L1-mediated acetylation prevents Rictor degradation in cardiac cells after hypoxic stress.

Human AC16 perturbations link GCN5L1 to Rictor acetylation, stability and signaling under hypoxic stress.

Reactome:R-HSA-429815

Formation Of Bloc-1 Complex

This event describes formation of BLOC-1.

PMID:16837549

BLOC-1 interacts with BLOC-2 and the AP-3 complex to facilitate protein trafficking on endosomes.

BLOC-1 facilitates AP-3/BLOC-2-associated cargo sorting and localizes to early-endosomal tubules.

PMID:19546860

The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth.

BLOC-1 deficiency impairs neurite outgrowth in cultured neonatal mouse hippocampal neurons.

PMID:21998198

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.

BLOC-1 and AP-3 support sorting of PI4KIIalpha from neuronal cell bodies to processes and terminals.