NCU06296: biological evidence
NCU06296 is a predicted flavin-containing monooxygenase with an FMO-like dinucleotide-binding architecture. Family evidence supports FAD-dependent oxygenation using a reduced nicotinamide cofactor, commonly NADPH. Its physiological substrate, reaction specificity, pathway, and cellular compartment in Neurospora crassa remain unknown.
- monooxygenase activity: The FMO-like catalytic architecture and conserved monooxygenase IBA support broad flavin-dependent oxygenation. Experimentally characterized fungal FMO establishes NADPH/O2-dependent oxidation, while the substrate remains unresolved for this target.
- N,N-dimethylaniline monooxygenase activity: IPR020946 identifies a flavin-monooxygenase-like family with diverse substrates; it does not uniquely identify N,N-dimethylaniline as the acceptor. The target lacks retrieved substrate assays or a resolved characterized substrate-specific subfamily, so this exact reaction cannot be confirmed or refuted.
- flavin adenine dinucleotide binding: FAD is the prosthetic flavin used by the supported FMO catalytic architecture. Family placement supports this cofactor-binding annotation without resolving the oxidized substrate.
- NADP binding: The FMO dinucleotide-binding architecture and experimentally characterized NADPH-dependent fungal FMO chemistry support conserved NADP(H) cofactor binding.
Primary evidence excerpts
- [file:NEUCR/NCU06296/NCU06296-uniprot.txt] “DR InterPro; IPR020946; Flavin_mOase-like.”
- PMID:10077572 “The flavin-containing monooxygenase from yeast (yFMO) catalyzes the O2- and NADPH-dependent oxidations of biological thiols, including oxidation of glutathione to glutathione disulfide (GSSG).”
Provenance: live API snapshot 2026-09-09T03:00:51.831347+00:00. Complete API prediction JSON and all emitted claim IDs, text, and original evidence are preserved in the source and provenance JSON files. Current sequence/annotation data are separate comparison snapshots. Annotation overlap records known biology, not demonstrated training membership. All seven gene-focused Falcon jobs completed; the provider reports were inspected and useful primary leads checked. Publication retrieval used Europe PMC metadata/XML when the canonical PubMed fetch returned HTTP 429.