Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic assignment of GO terms using logical inference, based on inter-ontology links
Network organization of the human autophagy system.
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ATG2B is a known autophagy protein in a human autophagy interaction network and associates with WDR45.
"association between ATG2A, ATG2B, and WDR45 was unaltered by mTOR inhibition"
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets.
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Mammalian ATG2A and ATG2B are required for autophagosome formation and also affect lipid droplet morphology.
"These data suggest that mammalian Atg2A and Atg2B function both in autophagosome formation and regulation of lipid droplet volume and distribution."
Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation.
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ATG2B forms a complex with WDR45/WIPI4, supporting ATG2-ATG18 complex membership.
"We also purified the ATG2B-WDR45 complex and then performed 3-dimensional reconstruction of the complex"
Human ATG2B possesses a lipid transfer activity which is accelerated by negatively charged lipids and WIPI4.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex AP-MS data provide proteome-scale interaction context for high-throughput protein-binding annotations.
"Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks."
Global Proximity Interactome of the Human Macroautophagy Pathway.
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BioID mapping places core macroautophagy proteins in a proximity-interaction network and supports cautious interpretation of high-throughput interaction annotations.
"Here, we applied BioID to the study of macroautophagy in human cells, generating a proximity interaction map of 39 core macroautophagy proteins."
OpenCell: Endogenous tagging for the cartography of human cellular organization.
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OpenCell provides endogenous tagging, localization, and interactome measurements for human cellular organization.
"well-curated localization and interactome measurements"
A conserved ATG2-GABARAP family interaction is critical for phagophore formation.
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A conserved ATG2 LIR mediates binding of ATG2A/ATG2B to GABARAP-subfamily ATG8 proteins; this interaction is required for phagophore formation and closure, whereas the ATG2-WIPI4 interaction is dispensable for autophagy flux.
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Endogenous ATG2B co-localizes with ATG2A on early autophagic membranes positive for WIPI2 and ATG16L1, providing direct ATG2B localization evidence at phagophore assembly sites.
ATG2 transports lipids to promote autophagosome biogenesis.
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ATG2 is a lipid-transfer protein operating at the ER-autophagosome interface; lipid transfer is the major autophagy-dependent activity, since a lipid-transfer-competent ATG2 N-terminal fragment rescues autophagosome biogenesis in ATG2A/ATG2B double-knockout cells whereas lipid-transport-defective mutants do not.
ANKFY1 bridges ATG2A-mediated lipid transfer from endosomes to phagophores.
Germline duplication of ATG2B and GSKIP predisposes to familial myeloid malignancies.
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An autosomal-dominant germline 14q32 duplication spanning ATG2B and GSKIP predisposes to familial myeloid malignancies, and overexpression of ATG2B/GSKIP enhances hematopoietic progenitor differentiation and cooperates with JAK2/MPL/CALR driver mutations; the disease effect is a dosage-sensitive locus-level property shared with GSKIP rather than an ATG2B-specific molecular function.