Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Three rhamnosyltransferases responsible for assembly of the A-band D-rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for the initiation of both A-band and B-band lipopolysaccharide synthesis.
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wbpX, wbpY and wbpZ mutants lack A-band LPS while B-band LPS is unaffected; WbpY was proposed by sequence and phenotype to add a1-3-linked D-Rha.
"these mutants are unable to synthesize A-band LPS, while B-band synthesis is unaffected"
Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY.
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Recombinant WbpY transfers one D-Rha from GDP-D-Rha in a1-3 linkage to the WbpZ product; WbpX and WbpY together make D-rhamnan oligomers non-processively.
"NMR spectroscopic analysis of the WbpY reaction product showed that WbpY transferred one D-Rha residue in α1-3 linkage"
Biosynthesis of the Common Polysaccharide Antigen of Pseudomonas aeruginosa PAO1: Characterization and Role of GDP-D-Rhamnose:GlcNAc/GalNAc-Diphosphate-Lipid α1,3-D-Rhamnosyltransferase WbpZ.
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WbpZ adds the first D-Rha to GlcNAc/GalNAc-PP-lipid, generating the acceptor used by WbpY.
"WbpZ transferred one D-Rha residue from GDP-D-Rha in α1-3 linkage to both GlcNAc- and GalNAc-diphosphate-lipid acceptor substrates"