SRP19 (P09132, human) review notes

Identity and overview

• UniProt P09132, SRP19_HUMAN, "Signal recognition particle 19 kDa protein", 144 aa, HGNC:11300, gene on chromosome 5.
• Member of the SRP19 family [file:human/SRP19/SRP19-uniprot.txt "Belongs to the SRP19 family"].
• Component of the signal recognition particle (SRP), a ribonucleoprotein that mediates cotranslational targeting of secretory/membrane proteins to the ER [file:human/SRP19/SRP19-uniprot.txt "Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER)"].
• SRP19 binds directly to 7SL (SRP) RNA and mediates binding of SRP54 to the SRP complex [file:human/SRP19/SRP19-uniprot.txt "Binds directly to 7SL RNA (By similarity). Mediates binding of SRP54 to the SRP complex (By similarity)."].

Core molecular function: SRP RNA binding / scaffolding required for SRP54 recruitment (S-domain assembly)

• SRP19 is a scaffolding protein that clamps helices 6 and 8 of SRP RNA, and this prepares the asymmetric loop of helix 8 for SRP54 binding PMID:27899666 PMID:27899666.
• In vitro reconstitution shows SRP19 must bind SRP RNA before SRP54 can bind ("SRP54-late" assembly), effecting a conformational change in the RNA that promotes SRP54 binding PMID:10618370 PMID:10618370.
• SRP19 is unstructured when free and folds (compact core + two extended RNA-binding loops) upon SRP RNA binding; SRP54 then binds the preformed SRP19-RNA complex forming a threefold RNA-protein interface PMID:17434535.
• SRP19 binds SRP RNA independently of other SRP proteins PMID:10618370.
• This is an RNA-binding/scaffolding function, NOT a GTPase. The GTPase of SRP is SRP54 (and the SRP receptor SRalpha); SRP19 is not a GTPase. PMID:27899666

GO term mapping

• 7S RNA binding (GO:0008312) is the most specific MF term for the SRP RNA-binding activity, directly supported by IDA in PMID:27899666 (CAFA) and PMID:17434535. RNA binding (GO:0003723) is the parent; HDA (PMID:22658674 mRNA interactome capture) and DisProt IPI (PMID:17434535) annotations are to this generic parent.
• signal recognition particle / SRP, ER targeting (GO:0048500, GO:0005786) — SRP19 is a constitutive subunit of the 6-protein + 7SL RNA SRP [file:human/SRP19/SRP19-uniprot.txt "Component of a signal recognition particle complex that consists of a 7SL RNA molecule of 300 nucleotides and 6 protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9"].
• SRP-dependent cotranslational protein targeting to membrane (GO:0006614) and the signal-sequence-recognition child (GO:0006617) and the older parent cotranslational protein targeting to membrane (GO:0006613) — all correct BP annotations.

Subcellular location

• Cytoplasm / cytosol (site of SRP function) [file:human/SRP19/SRP19-uniprot.txt "SUBCELLULAR LOCATION: Cytoplasm"]. Experimental cytoplasm annotations from PMID:10618370 and PMID:11682607.
• Nucleus, nucleolus and nucleoplasm — SRP partially assembles in the nucleus/nucleolus; GFP-SRP19 shows prominent nucleolar plus nucleoplasmic localization PMID:10618370 PMID:10618370. SRP19 is actively imported to the nucleus by importin 8 and transportin PMID:11682607 PMID:11682607.
• Nucleus/nucleolus are real but represent the assembly compartment, not the core ER-targeting site of action; keep as non-core localizations.

ribosome binding (GO:0043022, contributes_to, IDA PMID:27899666)

• SRP72-RBD remodels the 5f-loop "involved in ribosome binding" and the paper concerns SRP RNA remodeling/ribosome interaction; SRP19 is one component clamping the RNA. SRP as a whole binds ribosomes. The contributes_to qualifier is appropriate (SRP19 contributes to the complex's ribosome binding). Keep as non-core. PMID:27899666

protein binding (GO:0005515) IPI annotations

• PMID:33961781 (BioPlex / Huttlin dual proteome-scale network) and PMID:35271311 (OpenCell endogenous tagging) — both have WITH=UniProtKB:Q9UHB9 (SRP68). These capture the SRP19-SRP68 interaction within the SRP S domain. UniProt records this IntAct interaction: [file:human/SRP19/SRP19-uniprot.txt "P09132; Q9UHB9: SRP68; NbExp=6"]. Bare protein binding is uninformative per curation guidelines; keep as non-core (genuine interaction, but covered by SRP complex membership).

PMID:1678319 (familial polyposis locus)

• This is the original positional cloning paper that mapped SRP19 near the APC locus on chromosome 5; one of the new genes "contained sequence identical to SRP19, the gene coding for the 19 kd component of the ribosomal signal recognition particle" PMID:1678319. Used as the TAS source for SRP membership (GO:0005786) and cotranslational targeting (GO:0006613). The annotations are correct (SRP membership / cotranslational targeting), even though the paper's primary topic is the APC polyposis locus.

PMID:18089836 (TAS-103 drug target screen) — IDA for SRP membership (GO:0005786)

• This drug-target paper found SRP as the target of TAS-103; it identified the SRP54 subunit as the binding protein, and showed TAS-103 disrupts SRP complex formation and reduces the amount of SRP14 and SRP19 PMID:18089836. SRP19 is detected as part of the SRP complex here; supports SRP membership (part_of GO:0005786). Per guidelines (do not REMOVE an IDA just because the abstract foregrounds SRP54), accept the SRP-membership annotation.

PMID:34208095 (review, ComplexPortal NAS source)

• Review stating SRP19 functions to stabilize the 7SL structure: PMID:34208095. SRP is a six-protein + 7SL RNA complex with Alu and S domains; SRP19 binds in the S domain. Supports SRP membership and cotranslational targeting BP.

Summary of core vs non-core

• CORE MF: 7S RNA binding (GO:0008312) — SRP RNA binding/scaffolding required for SRP54 recruitment and S-domain assembly.
• CORE complex: signal recognition particle (GO:0048500 / GO:0005786, part_of).
• CORE BP: SRP-dependent cotranslational protein targeting to membrane (GO:0006614 and children GO:0006617/GO:0006613).
• Non-core: generic RNA binding (GO:0003723, parent of 7S RNA binding); ribosome binding (contributes_to, complex-level); protein binding (IPI to SRP68); nucleus/nucleolus/nucleoplasm/cytoplasm localizations (assembly + steady-state compartments, not the defining ER-targeting site).
• No GTPase function (SRP19 is not a GTPase).

Falcon deep-research findings (incorporated 2026-06)

• Foundational structure of the SRP19 RNA clamp: crystal structure of SRP19 bound to the S-domain of SRP RNA shows SRP19 clamps the tetraloops of helices 6 and 8 (helix 6 acting as a "splint") and pre-organizes the SRP54-binding site on helix 8 PMID:12086622. Added (HIGH) and used to strengthen the core 7S-RNA-binding/scaffolding core_function.
• Eukaryote-specific assembly hierarchy: unlike bacterial systems, human SRP54 cannot bind 7SL RNA before SRP19, placing SRP19 upstream as the assembly factor that gates SRP54 incorporation PMID:12086622. Added to the SRP54-incorporation core_function supported_by.
• Nucleolar assembly phase: GFP-SRP19 accumulates in nucleoli and co-localizes with nucleolar markers; nucleolar disruption (low-dose actinomycin D; uL18/RPL5 depletion) relocalizes SRP19, and SRP proteins associate with many nucleolar/ribosome-biogenesis factors PMID:38858088. Added (MEDIUM); reinforces the existing non-core nucleolar/nucleoplasm assembly localizations (already supported by PMID:10618370, PMID:11682607).
• Disease/autoantigen context: SRP19 is among the SRP autoantigens (anti-SRP19, anti-SRP54, anti-SRP72, anti-7SL) recognized in immune-mediated necrotizing myopathy (IMNM)/anti-SRP myositis PMID:35754847. Added (LOW); not a core molecular-function source.
• The Falcon "kellogg2023unravelingsrpbiogenesis" source is a 2023 thesis/dissertation ("Unknown journal"); its claims (SRP19 abundance depends on other SRP subunits; SRP54 depletion disrupts SRP19 ribosome association; ~500 SRP-dependent genes) are not separately PMID-resolvable, so notes-only (not added).
• A Falcon-cited flavivirus NS4B-SRP19 interaction (attributed to Wong et al. 2024 PLOS Pathogens, but the citation key actually points to the Kellogg thesis) could not be verified on PubMed; not added.