The saved replay joins all 58 imported annotations to their exact normal GOA contexts, including qualifiers, evidence, reference, donor, negation and product fields. The official Q13144 sequence is 721 residues and exactly matches the aligned target in the 218-node PANTHER tree.
Four positive IBD records at PTN000500978 lie on the target lineage and cover exchange activity, translation-initiation-factor activity, factor binding and complex membership. Donor counts and the target appearing among descendant evidence are not measures of circularity. The three ARBA rules match condition sets 20, 0 and 0 respectively; the replay retains the actual predicates. Complete saved donor queries contain 75 rat Q64350, 76 mouse Q8CHW4 and 26 rabbit P47823 records, separating experimental and propagated evidence.
The IntAct snapshot contains 228 records. Seven imported binding assertions are checked: five join to source-specific records, while the historical gamma/delta assertions have no current matching record and are assessed from their original complex experiments instead. The GSK3B records preserve the engineered epsilon peptide and modifications; the STX11, DYNC1H1 and KLK6 records preserve the screen/co-IP methods and partner identities. Partner reference checksums are not experimental insert sequences, and association is not automatically a native binary interface.
Five typed Reactome paths from R-HSA-72670 and R-HSA-72722 reach human EIF2B5 entity 72523, residues 2–721, in cytosol. This is a curated pathway model. The HPA target page independently reports enhanced Cytosol localization in six antibody/cell-line combinations: HPA064370 with MCF-7, PC-3 and U2OS, and HPA069303 with HaCaT, RT-4 and U2OS. No figure pixels were interpreted, and cytosol is not claimed to be exclusive.
These literal results support, but do not mechanically determine, the source-specific reviews. Original text access, disease/assay distinctions and unresolved mechanisms are documented in the source guide and source-locator inventory.