The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation.
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CupA3 is the usher of the CupA system, by similarity to E. coli FocD.
"CupA3 appears to be the usher component of the system, based on its high similarity to FocD"
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A cupA3 insertion mutant shows about 10% of parental attachment to an abiotic surface.
"This strain, carrying a disrupted cupA3 gene, showed an adhesion-deficient phenotype, with only 10% of the bacteria adhering relative to the parental PAK-NP"
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The usher forms an outer-membrane channel through which pilin subunits are assembled.
"called usher, that forms a channel into the outer membrane through which the pilin subunits are sequentially assembled"
Biofilm formation in Pseudomonas aeruginosa: fimbrial cup gene clusters are controlled by the transcriptional regulator MvaT.
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The cupA cluster is required for biofilm formation on abiotic surfaces; MvaT represses cupA.
"The cupA gene cluster, but not the cupB or cupC cluster, is required for biofilm formation on abiotic surfaces."
Responses of Pseudomonas aeruginosa to low oxygen indicate that growth in the cystic fibrosis lung is by aerobic respiration.
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
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The cupA operon is essential for SDS-induced aggregation.
"Transposon and site-directed mutagenesis revealed that the cupA-operon and the co-transcribed genes siaA (PA0172) and siaD (PA0169) were essential for SDS-induced aggregation."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Automatic assignment of GO terms using logical inference, based on on inter-ontology links.