putP (PA0783, Q9I5F5) review notes

Sodium/proline symporter, solute:sodium symporter family (TC 2.A.21.2.3;
NCBIfam TIGR02121 Na_Pro_sym; PANTHER PTHR48086). Unreviewed UniProt entry.
Multi-pass inner-membrane protein. Operon putA-putP, induced by proline via
the AraC/XylS activator PruR.

Literature (no deep-research provider available; own reading)

Nakada et al. 2002 (full text read). putA and putP form an operon; PruR
activates it in response to proline. A putP::ΩSp/Sm knockout grows poorly on
proline PMID:12270821, and the
authors conclude PutP is the major proline transporter PMID:12270821. PutP is 77% identical to E. coli PutP
PMID:12270821. No direct uptake assay is reported in this paper.
No other PAO1 PutP functional papers found. The function rests on this
genetics plus close homology to E. coli PutP, a well-studied Na+/proline
symporter (QuickGO: P07117 IDA/EXP GO:0005298).

PseudoCAP IBA-seed assessment

Other seeds' experimental support queried live in QuickGO on 2026-11-05.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN005155964	GO:0022857 transmembrane transporter activity	7	29 (21 taxa)	GO:0015193, IMP, PMID:12270821 (PseudoCAP)	SOUND	Knockout grows poorly on proline; authors conclude PutP is the main proline transporter; 77% identity to E. coli PutP. Descendant term supports the generic node term, which suits the SSS-family clade.	E. coli PutP P07117 (IDA GO:0005298), PanF P16256 (IDA GO:0015233), ActP P32705 (IDA GO:0015123), Salmonella PutP P10502 (EXP), B. subtilis PutP P94392 (CACAO IMP), C. albicans CGD:CAL0000179770	Yes; several IDA seeds
PTN001371338	GO:0015193 L-proline transmembrane transporter activity	2	20 (16 taxa)	same row	SOUND	as above; node is the PutP subfamily	B. subtilis PutP P94392 (CACAO IMP GO:0015193). The sibling IBD GO:0005298 on the same node rests on E. coli and Salmonella PutP (IDA/EXP).	Yes, on P94392; and the node's PutP members (P07117, P10502) are experimentally proline transporters

Note: in the current GO, GO:0005298 proline:sodium symporter activity is not
an is_a descendant of GO:0015193 L-proline transmembrane transporter activity,
so the E. coli/Salmonella IDA rows do not by themselves entail the GO:0015193
IBD; P94392 does. Not a defect.

No PSEUDOCAP defect and no GO_CENTRAL defect.