Megf10 (mouse) notes

Reviewed alongside human MEGF10 (genes/human/MEGF10/). The literature synthesis is in genes/human/MEGF10/MEGF10-notes.md. No provider deep research was run: Falcon returned 402 and the OpenAI key was invalid in this environment.

Mouse-specific evidence

• Glial engulfment of apoptotic DRG neurons: PMID:19915564. Also PMID:19915564.
• Astrocyte clearance in the cerebellum: PMID:27170117.
• Satellite cells: PMID:18056409.
• Synapse elimination by astrocytes: PMID:24270812.

GO:1902742 apoptotic process involved in development (IMP, MGI, PMID:27170117): REMOVE

The phenotype is more TUNEL- and cleaved-caspase-3-positive cells in the P7 cerebellum. The full text was read at PMC4863057; the local cache has only the abstract. Its Figure 1 title is "Megf10 is necessary for apoptotic cell uptake by astrocytes, and its deficiency results in accumulation of apoptotic cells in the developing CB". The authors therefore read the excess apoptotic cells as uncleared corpses, not extra death. The annotation turns a clearance defect into participation in apoptosis. The human ISS row inherits this error. Both reviews mark it REMOVE, and the case is logged in projects/APOPTOSIS/ASSAY_READOUT_OVERANNOTATION.md.

2026-10-12 Ortholog/family audit

• GOA refreshed: no change; all 53 GOA rows covered; NEW synapse pruning (GO:0098883, PMID:24270812) retained.
• Compared with genes/human/MEGF10 and genes/worm/ced-1 (no Drosophila drpr or MEGF11 review exists). Mouse and human actions agree term-for-term (scavenger receptor activity + C1q binding core; engulfment/recognition of apoptotic cell ACCEPT; muscle/satellite-cell terms non-core; homotypic cell-cell adhesion MODIFY to GO:0009988; vesicle-mediated transport and cell development over-annotated). PANTHER: mouse/human PTHR24052:SF13; worm ced-1 is PTHR24043 (scavenger receptor class F), consistent with CED-1 being the functional rather than strict PANTHER ortholog.
• Apoptotic process involved in development (GO:1902742): REMOVE in mouse (IMP; clearance-defect readout) and human (ISS), MODIFY to positive regulation in worm ced-1, where engulfment genes promote cell killing PMID:11449278. The difference reflects documented nematode biology; no change.
• Unresolved, human-side only: the human MEGF10 NEW synapse-pruning entry uses evidence_type IMP citing the mouse study PMID:24270812; ISS would describe the inference better. Not edited (not a donor to mouse).
• No changes made to the mouse review.