Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.
Involvement of microRNA in AU-rich element-mediated mRNA instability.
Importance of translation and nonnucleolytic ago proteins for on-target RNA interference.
Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.
The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.
The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins.
Defining the membrane proteome of NK cells.
ATP-dependent human RISC assembly pathways.
Slicing-independent RISC activation requires the argonaute PAZ domain.
Multivalent Recruitment of Human Argonaute by GW182.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
MIR449 microRNAs bind 3'UTR of NOTCH1 mRNA
MIR34 microRNAs bind 3'UTR of NOTCH1 mRNA
MIR150 microRNA binds 3'UTR of NOTCH3 mRNA
MIR200B/C microRNAs bind NOTCH1 mRNA
MIR181C microRNA binds 3'UTR of NOTCH4 mRNA
MIR206 microRNA binds 3'UTR of NOTCH3 mRNA
MIR34 microRNAs bind 3'UTR of NOTCH2 mRNA
MIR302A microRNA binds 3'UTR of NOTCH4 mRNA
p53 positively regulates transcription of MIR34 microRNAs
miR-26A microRNAs bind PTEN mRNA
miR-24 binds p16INK4A and p14ARF mRNAs
RISC binds inexactly matching target RNAs
Nonendonucleolytic RISC binds exactly matching target RNAs
miR-92b binds 3'UTR of NLK mRNA
FOXO3 regulates MIR34B,C expression
MIR27A gene transcription is stimulated by the complex containing RUNX1, PRMT1 and GATA1 and inhibited by the complex of RUNX1, SIN3A and PRMT6
miR-106a binds RUNX1 mRNA
miR-214 microRNA binds PTEN mRNA
miR-205 microRNA binds PTEN mRNA
miR-21 nonendonucleolytic RISC binds PTEN mRNA
miR-26A and B bind to the 3'UTR of the GREB1 mRNA
miR-26A and B bind to the 3'UTR of the CHD11 mRNA
miR-26A and B bind to the 3'UTR of the KPNA2 mRNA
miR-613 binds to the 3'UTR of the NR1H3 mRNA
miR-26 binds to the 3'UTR of the ARL4C mRNA
miR-26 binds to the 3'UTR of the ABCA1 mRNA
miR-33 binds to the 3'UTR of the ABCA1 mRNA
miR-144 binds to the 3'UTR of the ABCA1 mRNA
MITF-M-dependent miR-211 expression
miR-211 RISC binds POU3F2 mRNA
CD274 mRNA binds miR-34 RISC
CD274 mRNA binds miR-340 RISC
CD274 mRNA binds miR-152 RISC
CD274 mRNA binds miR-140 RISC
CD274 mRNA binds miR-200B/C RISC
UniProt text export for AGO4
GOA annotation export for AGO4
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GOA records the phylogenetic IBA source for the AGO4 GO:0004521 annotation.
"UniProtKB Q9HCK5 AGO4 enables GO:0004521 RNA endonuclease activity molecular_function ECO:0000318 IBA GO_REF:0000033 FB:FBgn0000146|FB:FBgn0004872|FB:FBgn0087035|FB:FBgn0250816|FB:FBgn0262739|MGI:MGI:1928897|MGI:MGI:1930036|MGI:MGI:2446632|PANTHER:PTN008584027|PomBase:SPCC736.11|TAIR:locus:2007760|TAIR:locus:2059258|UniProtKB:O67434|UniProtKB:Q9H9G7|UniProtKB:Q9UKV8|WB:WBGene00000105|WB:WBGene00000106|WB:WBGene00004323|WB:WBGene00017641 9606 Homo sapiens GO_Central Protein argonaute-4 20241120"
Falcon deep research report for AGO4
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Falcon research supports the reviewed core function of AGO4.
"Human AGO4 (EIF2C4; UniProt Q9HCK5) is best supported (in the retrieved literature set) as a **small-RNA-binding Argonaute paralog** that contributes to gene regulation largely through **non-slicing mechanisms**"
OpenScientist hypothesis report for AGO4 GO:0004521
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OpenScientist refutes RNA endonuclease activity for AGO4.
"Human AGO4 does **not** have RNA endonuclease (slicer) activity."
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The report identifies loss of the AGO2-equivalent catalytic Asp and His (AGO4 Gly671 and Arg809) and separately describes TNRC6-dependent translational repression and mRNA decay. Its conclusion concerns slicing, not all RNA processing or decay.
"AGO4's genuine molecular role is as a slicer-independent miRNA effector that silences targets by recruiting the TNRC6/GW182 machinery"
Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps.
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All four tethered human AGO proteins can accelerate biphasic deadenylation; AGO4 slicing inactivity does not imply absence of mRNA decay capacity.
"all four human Ago proteins and
TNRC6C are each able to recapitulate the two deadenylation steps."
OpenScientist AGO4 mRNA destabilization versus slicer-loss adjudication
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The report distinguishes artificial tethering sufficiency from natural guide-target recruitment.
"Tethering bypasses guide:target recognition, and paralogs differ quantitatively."