Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning, genomic organization, and characterization of a human cholinephosphotransferase.
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Identifies and clones human cholinephosphotransferase (hCPT1) and shows by in vitro enzyme assay and yeast reconstitution that it is a cholinephosphotransferase-specific enzyme making PC (not PE), unlike the dual-specificity paralog hCEPT1.
"codes for a cholinephosphotransferase-specific enzyme"
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hCPT1 and hCEPT1 differ in diradylglycerol specificity, including their capacity to synthesize platelet-activating factor and its precursor.
"capacity to synthesize platelet-activating factor and platelet-activating factor precursor"
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The cell-growth effect (yeast CPT1/SEC14 complementation) was a property of hCEPT1, not hCPT1.
"only hCEPT1-derived activity was able to complement the yeast CPT1 gene in its interaction with SEC14 and affect cell growth"
The major sites of cellular phospholipid synthesis and molecular determinants of Fatty Acid and lipid head group specificity.
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Localizes CPT1 to the Golgi (brefeldin A relocalization plus co-immunofluorescence and subcellular fractionation), distinct from CEPT1 which is ER/nuclear-membrane.
"confirmed that CPT1 was found in the Golgi and CEPT1 was found in both the endoplasmic reticulum and nuclear"
A reference map of the human binary protein interactome.
CDP-Cho and DAG are converted to PC by CHPT1 at the Golgi membrane
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Reactome reaction stating CHPT1 converts CDP-choline and DAG to PC and CMP at the Golgi membrane.
"At the Golgi membrane, cholinephosphotransferase 1 (CHPT1) converts CDP-choline (CDP-Cho) and diacylglycerol (DAG) to phosphatidylcholine (PC)"