Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
A reference map of the human binary protein interactome.
GPLD1 hydrolyses GPI-anchors from proteins
Organization, evolution and functions of the human and mouse Ly6/uPAR family genes.
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Mouse Ly6/uPAR family Table 3 lists LYPD2 with α4β2 nAChRs as the interacting factor and "nAChR Modulator" as the cellular function; a human ortholog exists and a GPI anchor is annotated "Yes".
"LYPD2YesUnknownα4β2 nAChRsnAChR ModulatorNoYes"
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LYPD2 is part of the chromosome 8q24 LY6 cluster of Ly6/uPAR family genes.
"Twelve human Ly6 genes are clustered together within a short span of about 500 kb on chromosome 8 (8q24)"
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Family-level mechanism for Ly6/uPAR proteins typically involves targeting nicotinic acetylcholine receptors (nAChRs).
"Commensurate with their varied expression patterns, Ly6/uPAR proteins have a wide range of functions in cell proliferation, migration, cell-cell interaction, immune cell maturation, macrophage activation, and cytokine production. They typically exert their influence by targeting nicotinic acetylcholine receptors (nAChRs)"
Human LY6 gene family: potential tumor-associated antigens and biomarkers of prognosis in uterine corpus endometrial carcinoma.
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The 8q24.3 LY6 cluster is enumerated and includes LYPD2 alongside LY6E, LY6L, LY6D, LY6K, LY6H, SLURP1, LYNX1, GML, and GPIHBP1.
"Genes located at this locus include LY6E, LY6L, LY6D, LY6K, LY6H, SLURP1, LYPD2, LYNX1, GML, and GPIHBP1; these genes are syntenic to mouse chromosome 15."
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LYPD2 mRNA shows no significant change between normal uterine tissue and uterine corpus endometrial carcinoma (UCEC).
"There is no significant change in mRNA expression for LYPD8, LY6G6D, LYPD4, LY6L, LYPD2, LYPD5, LY6G6F, LYPD4, GPIHBP1, and GML."
GluR2Q and GluR2R AMPA Subunits are not Targets of lypd2 Interaction.
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Co-IP in HEK-293 cells showed mouse lypd2 does not interact with homomeric GluR2R or GluR2Q AMPA receptors, providing negative evidence that narrows (but does not refute) the broader receptor- modulator hypothesis space for Ly6 proteins.
"The results of our experiments showed that lypd2 does not interact with homomeric GluR2R or GluR2Q AMPA receptors."
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Family-level context - Ly6 proteins are typically GPI-anchored to the outer leaflet of the plasma membrane.
"Ly6 proteins are generally found within the extracellular space, either as a secreted protein or (in the majority of cases) by being physically tethered to the outer leaflet of the plasma membrane by a post-translational C-terminal GPI anchor modification"
Glycosylphosphatidylinositol biosynthesis functions as a conserved host defense pathway against coronaviruses via regulation of LY6E.
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In focused CRISPR knockout screens of known or predicted GPI-anchored proteins, LYPD2 was among the top-10 enriched genes in all four coronavirus infection conditions. LY6E (not LYPD2) was the lead validated antiviral effector; LYPD2 itself was not individually validated in this paper.
"Among the top 10 enriched genes identified for each infection condition, LYPD2 was also identified in all four screens (Fig 6C)."
Deep research on LYPD2 function
Falcon deep research on LYPD2 - synthesis of LY6/uPAR family literature, GTEx expression, scRNA-seq marker usage, and CRISPR screen evidence.
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Synthesis concludes LYPD2 is very likely a cell-surface LU-domain protein, plausibly GPI-anchored and extracellular-facing by family analogy; reproducible signals are as a marker of specific cell states (esophagus enrichment; non-classical monocyte subset); mechanistic function (binding partners, pathway roles) remains a high-priority experimental gap.
"From a functional-annotation standpoint, the most defensible expert synthesis based on current evidence is: 1) LYPD2 is very likely a cell-surface LU-domain protein; 2) it is plausibly GPI-anchored and extracellular-facing by family analogy; 3) its most reproducible "functional" signal in recent data is as a marker of specific cell states/tissues (esophagus enrichment; non-classical monocyte subset); 4) mechanistic function (binding partners, pathway roles) remains a high-priority experimental gap."