ego-1 (G5EBQ3) review notes

Provenance / process

• Data fetched with just fetch-gene worm G5EBQ3 --alias ego-1 (TrEMBL G5EBQ3, 1632 aa, WormBase F26A3.3; same accession as modules/c_elegans_mutator_22g_rna_amplification.yaml).
• Deep research (just deep-research-falcon worm ego-1 --fallback perplexity-lite) FAILED: falcon timed out (exit 137) and the perplexity fallback provider is not configured. No deep-research file was written. The review relies on cached publications and PubMed searches.

Key findings

• EGO-1 is an RdRP-family protein required for germline development and for RNAi of germline genes PMID:10704412.
• It produces mRNA-templated triphosphorylated antisense small RNAs PMID:21396820. No direct in vitro RdRP assay of EGO-1 was found; RRF-1 accounted for most RdRP activity in the Aoki cell-free system PMID:18007599.
• RdRP complexes contain DRH-3 PMID:19800275.
• CSR-1 pathway: EGO-1 is on chromosomes and needed for segregation PMID:19804758.
• Meiotic silencing: PMID:16271877.
• EGO-1 may make some 22G-RNAs independently of pUG RNAs PMID:32499657.

Curation decisions

• Nuclear pore localization and P granule assembly (IMP) were marked as over-annotated. They are indirect consequences, because EGO-1 targets include nuclear pore assembly genes PMID:21396820.
• Germ cell development, oogenesis and spermatogenesis were kept as non-core.
• For the module curator: EGO-1 is mainly the CSR-1 22G-RNA RdRP, and the module's contrast with Mutator/RRF-1 is consistent with the literature. It also contributes partially to WAGO 22G-RNAs.