Gene Ontology annotation through association of InterPro records with GO terms

InterPro2GO assigns TOM complex membership based on the Tom5 family signature.

Annotation inferences using phylogenetic trees

PAINT/IBA phylogenetic propagation supports TOM complex membership for TOMM5 across Tom5 orthologs.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt subcellular location vocabulary maps TOMM5 to the mitochondrial outer membrane.

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

Curated immunofluorescence data place TOMM5 in mitochondria.

PMID:18331822

Identification of Tom5 and Tom6 in the preprotein translocase complex of human mitochondrial outer membrane.

Human Tom5 was immuno-isolated as part of the human TOM preprotein translocase complex from HeLa cells.

"We immuno-isolated the TOM complex from HeLa cells expressing hTom22-FLAG and identified the human counterparts of Tom5 and Tom6, together with the other components including Tom7."
Tom5 is associated with Tom40 in the human TOM complex.

"These small Tom proteins are associated with Tom40 in the TOM complex."
Knockdown of Tom7, but not Tom5 or Tom6, strongly compromises TOM complex stability; double knockdown of any small-Tom combination affects preprotein import, supporting a structural-integrity role for the small TOM subunits.

"Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex."

PMID:34800366

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Quantitative MS-based human mitochondrial proteome supports mitochondrial localization of TOMM5.

"Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context."

Reactome:R-HSA-5205661

Pink1 is recruited from the cytoplasm to the mitochondria

Reactome places TOMM5 at the mitochondrial outer membrane as a TOM complex subunit in the PINK1 recruitment pathway.

PMID:40080546

Structure of human PINK1 at a mitochondrial TOM-VDAC array.

In the endogenous TOM-VDAC array structure, TOM5 (TOMM5) is part of the TOM core complex and facilitates the symmetric arrangement of two TOM core complexes around a central VDAC2 dimer.

"TOM core complexes around a central VDAC2 dimer is facilitated by TOM5 and"

file:human/TOMM5/TOMM5-deep-research-falcon.md

Falcon deep research report for human TOMM5

TOMM5 is a single-pass α-helical small Tom subunit of the mitochondrial TOM translocase, not an independent enzyme or transporter.

"encodes the human homolog of the TOM-complex small subunit **Tom5**, a **single-pass α-helical** protein that is part of the mitochondrial **translocase of the outer membrane (TOM) complex**"
TOMM5 is an accessory structural/regulatory subunit supporting TOM complex stability, organization, and biogenesis around Tom40.

"TOMM5 is best understood as a **structural/regulatory accessory subunit** of a large membrane translocase, contributing to the assembly, stability, and/or mechanistic routing properties of the TOM pore"
In the human TOM core structure, Tom5 is single-pass α-helical and sits at the periphery of each Tom40 barrel together with Tom6 and Tom7.

"Tom5 (TOMM5) as a **single-pass α‑helical** subunit that **surrounds** the Tom40 β‑barrel together with Tom6 and Tom7"
The N-terminal segment of Tom40 traverses the channel and interacts with Tom5 at the periphery of the TOM dimer, a site implicated in recruitment of factors for presequence-lacking precursors.

"the **N‑terminal segment of Tom40** traverses the channel and **interacts with Tom5** at the **periphery** of the TOM dimer"
Yeast ortholog evidence indicates Tom5 associates with the Tom40 precursor at the SAM complex during late TOM assembly, supporting a conserved role in TOM core biogenesis.

"**Tom5 associates with the Tom40 precursor at the SAM complex** in a later stage that promotes Tom40 assembly"