The primary purified-enzyme abstract PMID3282541 explicitly reports activation by zinc, not ferrous ions. Family group III naming does not identify the native cofactor. PMID17938904 reports zinc-limited industrial-yeast compensation associated with Adh4 upregulation but calls it Fe-dependent without a direct metal-reconstitution assay in the accessible abstract. PMID22094012 finds Q4 fails to use ethanol and does not attribute its modest ethanol production confidently to Adh4. PMID3023838 identifies activating Ty insertions at ADH4 in adh1 mutants selected for low-temperature antimycin resistance. Therefore standard-culture weak contribution is conditional, not proof of catalytic incapacity. Cytosolic reaction compartment remains UNDECIDED despite mitochondrial localization; pathway projection alone is insufficient. Ehrlich reduction remains supported by combinatorial mutants (PMID12499363).
Workflow: fetched current gene data using just fetch-gene; existing original row fields were preserved (verified against the pre-edit JSON snapshot). All 43 rows were reassessed by term and source, including non-fermentation activities/localizations. Annotation-reviewer and core-function synthesis instructions were applied. just deep-research-falcon yeast ADH4 --fallback perplexity-lite ran concurrently with just fetch-gene-pmids: Falcon/Edison failed HTTP403 authentication; Perplexity failed HTTP401 insufficient_quota. No generated provider report was fabricated. Publication caching completed successfully. FEBA fitness is a prokaryote-only review requirement and does not apply to yeast.
Family membership from the fetched record (official names independently checked in interpro/panther/panther.obo):
DR PANTHER; PTHR11496; ALCOHOL DEHYDROGENASE; 1.
DR PANTHER; PTHR11496:SF102; ALCOHOL DEHYDROGENASE 4; 1.
GO-CAM index lookup by the UniProt accession returned no entries in the current index; existing RCA/YeastPathways assertions were assessed from their explicit terms and underlying chemistry. No new process rows were manufactured.
Audit decisions by original term (duplicate evidence rows remain intact):