Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Ethanolamine Catabolism in Pseudomonas aeruginosa PAO1 Is Regulated by the Enhancer-Binding Protein EatR (PA4021) and the Alternative Sigma Factor RpoN.
-
PA4022 is the first gene of the PA4022-eat-eutBC ethanolamine-catabolic operon and is assigned the acetaldehyde dehydrogenase step; the operon is induced by acetaldehyde via EatR and RpoN.
"This pathway is encoded by the PA4022-eat-eutBC operon and consists of a transport protein (Eat), an ethanolamine-ammonia lyase (EutBC), and an acetaldehyde dehydrogenase (PA4022)."
Cytochrome c550 is an essential component of the quinoprotein ethanol oxidation system in Pseudomonas aeruginosa: cloning and sequencing of the genes encoding cytochrome c550 and an adjacent acetaldehyde dehydrogenase.
-
The ATCC17933 ExaC comparator is an NAD-dependent acetaldehyde dehydrogenase; it is distinct from both PAO1 PA4022 and PA1984.
"An ORF encoding a NAD(+)-dependent acetaldehyde dehydrogenase (exaC) was located downstream of the cytochrome c550 gene and in the same orientation."
UniProtKB entry for Pseudomonas aeruginosa ExaC
UniProtKB entry for Pseudomonas aeruginosa PAO1 PA4022 (Q9HX05)
Pairwise comparison of PA4022 with ExaC (Q9ZAA1) and PAO1 exaC (PA1984)
Group X aldehyde dehydrogenases of Pseudomonas aeruginosa PAO1 degrade hydrazones.
-
Native PAO1 HdhA was identified as PA4022; recombinant HdhA and PA1984 ExaC directly oxidize acetaldehyde.
"We found here that purified rHdhA and rExaC both oxidize acetaldehyde."
-
Hydrazone oxidative hydrolysis yields hydrazides and acetate, rather than aldehydes from simple hydrolysis.
"The purified HDH converted AEH and NAD + to stoichiometric amounts of ADH, AMH, and acetate"
PAO1 primary growth and assay scope from PMID27325678
-
PA4022 deletion impairs ethanolamine carbon growth; PAO1 Eat deletion is dispensable under the tested conditions.
"Deletion of the PA4022 gene reduced the growth of P. aeruginosa PAO1 on ethanolamine as a sole source of carbon"
-
The later study did not reproduce hydrazone-dependent growth.
"we did not observe growth on hydrazone for any of the P. aeruginosa strains used in this study."