Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules.
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TIA-1 and TIAR recruit untranslated mRNAs to mammalian stress granules downstream of stress-induced eIF2-alpha phosphorylation.
"TIA-1 and TIAR act downstream of the stress-induced phosphorylation of eIF-2α to promote the recruitment of untranslated mRNAs to SGs."
Identification and functional characterization of a TIA-1-related nucleolysin.
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Recombinant TIAR induces DNA fragmentation in permeabilized target cells.
"Like TIA-1, purified recombinant TIAR induced DNA fragmentation in permeabilized target cells."
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A lysosome-targeting motif led the authors to propose cytotoxic-granule association.
"The carboxyl terminus of TIAR contains a lysosome-targeting motif, indicating that TIAR is probably a cytotoxic granule-associated protein."
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
An alternative form of nucleolysin binds to a T-cluster DNA in the silencer element of platelet factor 4 gene.
Identification of TIAR as a protein binding to the translational regulatory AU-rich element of tumor necrosis factor alpha mRNA.
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TIAR is a component of the cytosolic protein complex bound to the TNF-alpha AU-rich element.
"Here, we report the identification of the RNA-binding protein TIAR as a protein involved in complex 1."
Novel DNA-binding properties of the RNA-binding protein TIAR.
Translational repression by RNA-binding protein TIAR.
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TIAR binds 3-prime UTRs of translation-factor mRNAs and represses their translation.
"TIAR bound the 3'-untranslated regions of these mRNAs and potently suppressed their translation"
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TIAR silencing relieves UVC-induced global translation inhibition.
"The UVC-imposed global inhibition of the cellular translation machinery was significantly relieved after silencing of TIAR expression."
Posttranscriptional derepression of GADD45alpha by genotoxic stress.
Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing.
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TIA1 and TIAR promote suboptimal 5-prime splice sites followed by U-rich intronic enhancers.
"TIA-1 (T-cell intracellular antigen 1) and TIAR (TIA-1-related) proteins regulate alternative pre-mRNA splicing by promoting the use of suboptimal 5' splice sites followed by uridine-rich intronic enhancer sequences."
Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein.
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TIAR depletion changes endogenous TIA1 alternative isoform output in human and mouse cells.
"TIAR depletion from HeLa and mouse embryonic fibroblasts results in an increased ratio of TIA-1b/a expression"
DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization.
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Architecture of the human interactome defines protein communities and disease networks.
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BioPlex 2.0 produced a large candidate human protein co-association network.
"With more than 56,000 candidate interactions"
Rox8 promotes microRNA-dependent yki messenger RNA decay.
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Human TIAR can promote yki degradation in flies and destabilize YAP mRNA in human cells.
"TIAR, the human ortholog of Rox8, is able to promote the degradation of yki mRNA when introduced into Drosophila and destabilizes YAP mRNA in human cells."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex 3.0 generated cell-line-specific human protein co-association networks.
"These networks model the interactome whose structure encodes protein function, localization, and complex membership."
MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity.
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Endogenous MOV10 and TIAR co-immunoprecipitate even in uninfected human cells.
"An interaction between endogenous MOV10 and the SG component TIAR was also identified, even in mock-infected cells."
ESRP1 and 2 bind FGFR2 pre-mRNA to promote FGFR2b maturation and expression
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Reactome includes TIAL1 among nucleoplasmic factors contributing to FGFR2 IIIb-specific splicing.
"Other factors that appear to contribute to IIIb-specific splicing include hnRNPM, TIA1 and TIAL1"
UniProtKB record for human TIAL1 (Q01085)
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TIAL1 is a reviewed 375-amino-acid protein.
"Reviewed; 375 AA."
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UniProt assigns three RNA recognition motifs.
"FT DOMAIN 9..85"
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UniProt records nuclear, cytoplasmic, cytolytic-granule, and stress-granule localization.
"Cytoplasm, Stress granule"
Manual literature and annotation review notes for human TIAL1
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All 35 grouped GOA annotations were manually adjudicated.
"## Existing annotation decisions"
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The notes distinguish core RNA functions from historical candidate nucleolysin claims.
"## Cytolytic-granule, lysosome, defense, and apoptosis boundary"
Manual literature synthesis for human TIAL1 (Q01085)
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The best-supported core is U-rich RNA recognition coupled to splicing, translation control, and stress-responsive mRNP partitioning.
"The best-supported core picture is therefore a U-rich RNA recognition protein"