Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Multiple potential germ-line helicases are components of the germ-line-specific P granules of Caenorhabditis elegans.
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GLH-1 and GLH-2 are putative germline RNA helicases with retroviral-type CCHC zinc fingers
"Both components are putative germ-line RNA helicases (GLHs) that contain CCHC zinc fingers of the type found in the RNA-binding nucleocapsid proteins of retroviruses."
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GLH-2 has six CCHC zinc fingers (GLH-1 has four)
"The predicted GLH-1 protein has four CCHC fingers; GLH-2 has six."
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Both GLH proteins localize to P granules at all stages of germline development
"Both GLH proteins localize in the P granules at all stage of germ-line development."
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Antisense glh-1 or glh-2 causes sterility in some offspring, indicating a requirement for normal germline development
"Injection of antisense glh-1 or glh-2 RNA into wild-type worms causes some offspring to develop into sterile adults, suggesting that either or both genes are required for normal germ-line development."
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glh-1 and glh-2 map within a few hundred kb and likely arose from a recent duplication
"As these very similar glh genes physically map within several hundred kilobases of one another, it seems likely that they represent a fairly recent gene duplication event."
The GLH proteins, Caenorhabditis elegans P granule components, associate with CSN-5 and KGB-1, proteins necessary for fertility, and with ZYX-1, a predicted cytoskeletal protein.
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The GLHs are a family of four germline RNA helicases that localize to cytoplasmic P granules
"The GLH proteins belong to a family of four germline RNA helicases in Caenorhabditis elegans."
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GLHs bind KGB-1, a JNK-family MAP kinase, confirmed by GST pull-down
"KGB-1 is a putative JNK MAP kinase that GLHs bind."
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Three GLH interactors (CSN-5, KGB-1, ZYX-1) were identified and confirmed to bind GLHs
"GST pull-down assays independently established that these proteins bind GLHs."
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Loss of CSN-5 or KGB-1 stops oogenesis (like loss of GLH-1/GLH-4) but not initial P-granule assembly
"Similar to the loss of GLH-1 and GLH-4, loss of either CSN-5 or KGB-1 causes oogenesis to cease, but does not affect the initial assembly of P granules."
Genetic analysis of the Caenorhabditis elegans GLH family of P-granule proteins.
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C. elegans has four Vasa-family germline helicases, GLH-1 to GLH-4
"In contrast to the single Vasa gene in most systems analyzed, Caenorhabditis elegans has four Vasa family members, the germline helicases GLH-1, GLH-2, GLH-3, and GLH-4."
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Deletion-allele analysis shows GLH-1 is the key family member for fertility
"Our analysis of deletion alleles of each glh gene demonstrates that GLH-1 is the key member of the family"
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GLH-2, GLH-3 and GLH-4 are not individually essential
"The other GLHs are not essential."
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GLH-1 and GLH-4 are required for proper association of PGL proteins with P granules (GLHs upstream of PGLs)
"GLH-1 and GLH-4 are required for proper association of the PGL family of proteins with P granules"