Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
A missense mutation in the nuclear gene coding for the mitochondrial aspartyl-tRNA synthetase suppresses a mitochondrial tRNA(Asp) mutation.
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The nuclear suppressor NSM3 of a mitochondrial tRNA(Asp) acceptor-stem mutation is an MSD1 allele carrying S388N in the GAD insertion domain; the substitution alone is sufficient for respiratory rescue at single copy.
"In this work, one copy of a mutated version of the gene coding for mitochondrial AspRS per haploid cell was sufficient to recover the mitochondrial function."
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MSD1 activity is required for maintenance of the mitochondrial genome (a secondary consequence of lost mitochondrial translation).
"The MSD1 gene activity is required for maintenance of the mitochondrial genome."
The proteome of Saccharomyces cerevisiae mitochondria.
Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.
Quantitative variations of the mitochondrial proteome and phosphoproteome during fermentative and respiratory growth in Saccharomyces cerevisiae.
Homology of aspartyl- and lysyl-tRNA synthetases.
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MSD1 encodes mitochondrial AspRS; mitochondria from an msd1 disruptant cannot acylate mitochondrial aspartyl-tRNA, and the disruptant shows a mitochondrial protein-synthesis defect.
"Mitochondria from a mutant with a disrupted chromosomal copy of MSD1 are unable to acylate mitochondrial aspartyl-tRNA."
Functional analysis of missense DARS2 variants in siblings with leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation.
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msd1 null yeast cannot grow on non-fermentable carbon sources; wild-type MSD1 rescues, and MSD1 alleles modelling DARS2 disease variants (E259K, Q137V) fail to rescue fully without reducing Msd1 protein levels.
"MSD1-knock out yeast (msd1Δ) transformed with the empty vector exhibited complete inability to grow on the media containing non-fermentable carbon sources such as acetate, glycerol and lactate"
UniProt entry P15179 (MSD1)