UniProtKB record for human CXCL8 (P10145)
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Regulation of the human chemokine receptor CCR1. Cross-regulation by CXCR1 and CXCR2.
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Activation of CXCR1 or CXCR2 by IL-8 induces receptor internalization and heterologous desensitization of CCR1.
"Interleukin-8 stimulation of CXCR1 or CXCR2 cross-phosphorylated CCR1 and cross-desensitized its ability to stimulate GTPase activity and Ca(2+) mobilization."
Differential ability of exogenous chemotactic agents to disrupt transendothelial migration of flowing neutrophils.
Inflammation in the developing human intestine: A possible pathophysiologic contribution to necrotizing enterocolitis.
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IL-8 is secreted by fetal and newborn intestinal epithelial cells as part of the acute inflammatory response.
"Again in human organ cultures from fetuses compared to older children, IL-8 secretion was greater (LPS, 2.5-fold; IL-1beta, 200-fold) and mRNA activity after stimulation was comparably higher, suggesting that increased transcription of the IL-8 gene may account for the excessive response."
Involvement of alpha-2-macroglobulin receptor in clearance of interleukin 8-alpha-2-macroglobulin complexes by human alveolar macrophages.
Autocrine growth effect of IL-8 and GROalpha on a human pancreatic cancer cell line, Capan-1.
Interleukin-8 stimulates human immunodeficiency virus type 1 replication and is a potential new target for antiretroviral therapy.
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IL-8 is a pro-inflammatory chemokine that stimulates HIV-1 replication in macrophages and T lymphocytes via CXCR1 and CXCR2.
"Here we demonstrate that IL-8 also stimulates HIV-1 replication in macrophages and T lymphocytes."
Immobilized IL-8 triggers progressive activation of neutrophils rolling in vitro on P-selectin and intercellular adhesion molecule-1.
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Immobilized IL-8 activates rolling human neutrophils and promotes firm adhesion via ICAM-1 and beta2-integrins under shear flow.
"Neutrophils rolled an average of 200 microm on surfaces of P-sel, ICAM-1, and IL-8 before firmly adhering through ICAM-1-beta(2) integrin interactions at 2 dynes/cm(2) wall shear stress."
Ca(2+) signalling by recombinant human CXCR2 chemokine receptors is potentiated by P2Y nucleotide receptors in HEK cells.
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Both 72-amino-acid and 77-amino-acid isoforms of human IL-8 mobilize intracellular calcium through human CXCR2.
"1. Human embryonic kidney (HEK)-293 cells expressing recombinant G alpha(i)-coupled, human CXC chemokine receptor 2 (CXCR2) were used to study the elevation of the intracellular [Ca(2+)] ([Ca(2+)](i)) in response to interleukin-8 (IL-8) following pre-stimulation of endogenously expressed P2Y1 or P2Y2 nucleotide receptors."
An alternatively spliced variant of CXCR3 mediates the inhibition of endothelial cell growth induced by IP-10, Mig, and I-TAC, and acts as functional receptor for platelet factor 4.
Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES.
Structure and functional expression of a human interleukin-8 receptor.
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Cloned human IL-8 receptor (CXCR1) binds IL-8 with high affinity and mediates transient intracellular calcium mobilization.
"Mammalian cells transfected with the IL-8 receptor cDNA clone bind IL-8 with high affinity and respond specifically to IL-8 by transiently mobilizing calcium."
CXCL8/IL-8 and CXCL12/SDF-1alpha co-operatively promote invasiveness and angiogenesis in pancreatic cancer.
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CXCL8 enhances endothelial cell invasion and capillary tube formation in human umbilical vein endothelial cells.
"CXCL8 enhanced proliferation/invasion of HUVECs but did not promote proliferation/invasion of PaCa. Both recombinant and PaCa-derived CXCL8 enhanced tube formation of HUVECs that were co-cultured with fibroblast cells."
A20 is an early responding negative regulator of Toll-like receptor 5 signalling in intestinal epithelial cells during inflammation.
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Intestinal epithelial cells rapidly induce A20 and IL-8 upon bacterial flagellin or Salmonella stimulation.
"A20 expression increased rapidly and peaked at 1 h after flagellin stimulation in cultured IECs, then declined gradually to the basal level."
Chemical synthesis, purification, and characterization of two inflammatory proteins, neutrophil activating peptide 1 (interleukin-8) and neutrophil activating peptide.
Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma.
TGF-β2 suppresses macrophage cytokine production and mucosal inflammatory responses in the developing intestine.
HIV-1 matrix protein p17 promotes angiogenesis via chemokine receptors CXCR1 and CXCR2.
Inhibition of tumor angiogenesis and growth by a small-molecule multi-FGF receptor blocker with allosteric properties.
TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8.
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CXCL8 binds heparin and heparan sulfate glycosaminoglycans to drive neutrophil transendothelial migration, an interaction antagonized by TSG-6.
"Here we have demonstrated that TSG-6 acts to inhibit CXCL8-induced trans-endothelial migration of human neutrophils via a direct interaction between the TSG-6 Link module and CXCL8, which antagonizes the binding of CXCL8 to heparin/HS."
Cutting edge: GPR35/CXCR8 is the receptor of the mucosal chemokine CXCL17.
Mycobacterium tuberculosis AtsG (Rv0296c), GlmU (Rv1018c) and SahH (Rv3248c) Proteins Function as the Human IL-8-Binding Effectors and Contribute to Pathogen Entry into Human Neutrophils.
Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
MicroRNA-520c-3p negatively regulates EMT by targeting IL-8 to suppress the invasion and migration of breast cancer.
Structure of monomeric Interleukin-8 and its interactions with the N-terminal Binding Site-I of CXCR1 by solution NMR spectroscopy.
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Solution NMR structure of monomeric CXCL8 (1-66) defines its interaction with the N-terminal binding site-I of CXCR1.
"The structure of monomeric human chemokine IL-8 (residues 1-66) was determined in aqueous solution by NMR spectroscopy."
Structural basis of CXC chemokine receptor 2 activation and signalling.
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Cryo-EM structures of CXCL8-activated human CXCR2 bound to heterotrimeric Gi reveal the mechanism of receptor activation.
"Here we present the cryo-electron microscopy structures of interleukin-8 (IL-8, also known as CXCL8)-activated human CXC chemokine receptor 2 (CXCR2) in complex with Gi protein, along with a crystal structure of CXCR2 bound to a designed allosteric antagonist."
The chemokines CXCL8 and CXCL12: molecular and functional properties, role in disease and efforts towards pharmacological intervention.
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CXCL8 signals through CXCR1 and CXCR2 and interacts with ACKR1 and glycosaminoglycans.
"CXCL8 activity inherently depends on interaction with the human CXC chemokine receptors CXCR1 and CXCR2, the atypical chemokine receptor ACKR1, and glycosaminoglycans."
Structural basis of CXC chemokine receptor 1 ligand binding and activation.
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Cryo-EM structure of the CXCL8-CXCR1-Gi complex defines the structural basis of CXCR1 activation by CXCL8.
"Here, we solve the structure of CXCR1 complexed with CXCL8 and cognate G-proteins using cryo-EM, showing the detailed interactions between the receptor, the chemokine and Gαi protein."
IL-8 induces the locomotion of human IL-2-activated natural killer cells. Involvement of a guanine nucleotide binding (Go) protein.
Human and bovine granulocyte chemotactic protein-2: complete amino acid sequence and functional characterization as chemokines.
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IL-8 is a C-X-C chemokine that induces neutrophil chemotaxis and activates post-receptor signaling leading to gelatinase B release.
"Both chemokines can also activate postreceptor mechanisms leading to release of gelatinase B by granulocytes."
Lymphocyte-derived cytokines induce sequential expression of monocyte- and T cell-specific chemokines in human mesangial cells.
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TNF-alpha, IL-1beta, and bFGF stimulate IL-8 expression in human mesangial cells.
"Finally, unlike other mesangial growth factors basic fibroblast growth factor (bFGF) induced MCP-1, RANTES, and IL-8 mRNA expression, suggesting an involvement of autocrine regulation mechanisms in mesangial chemokine expression."
CXC chemokines suppress proliferation of myeloid progenitor cells by activation of the CXC chemokine receptor 2.
Changes in levels of serum IL-8 in burned patients.
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Soluble IL-8 is secreted into human serum and rises markedly after burn injury.
"In conclusion, the results showed that there was an increase in serum IL-8 postburn. Serum IL-8 was significantly higher in the septic patients, who all died."
Receptor CXCR1 binds CXCL6 and CXCL8 ligands
Receptor CXCR2 binds ligands CXCL1 to 7
DARC is a non-specific receptor for many chemokines
Stimulation of IL8 transcription in senescent cells
Liganded Gi-activating GPCR acts as a GEF for Gi
IL10 negatively regulates extracellular inflammatory mediators
Expression of IL4,IL13-downregulated extracellular genes
The Ligand:GPCR:Gi complex dissociates
Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi