Annotation inferences using phylogenetic trees
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NSG2 IBA annotations are family-transfer annotations from the INSIG/PANTHER context and should be interpreted as conserved regulatory/chaperone functions, not sterol-enzyme activity.
"GOA WITH/FROM includes PANTHER:PTN000393022 for ER and sterol biosynthetic process annotations."
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
UniProtKB entry for NSG2_YEAST
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Nsg2 is an ER membrane INSIG-family protein.
"FUNCTION: Stabilizes the HMG-CoA reductase HMG2 by preventing its HRD1-dependent degradation. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the INSIG family."
Global analysis of protein localization in budding yeast
INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins
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Nsg1 and Nsg2 are yeast INSIG homologs that regulate Hmg2 stability through direct interaction with its SSD-containing transmembrane region.
"We now show that the yeast INSIG homologs NSG1 and NSG2 function to control the stability of yeast Hmg2p, the HMGR isozyme that undergoes regulated ubiquitination."
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The NSG proteins are best interpreted as dedicated chaperones for SSD-containing client proteins.
"One way to unify the known, disparate actions of INSIGs is to view them as known adaptations of a chaperone dedicated to SSD-containing client proteins."
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy
Role of Pex31 in metabolic adaptation of the nucleus-vacuole junction
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Nsg2 is a conditional nucleus-vacuole junction resident during low-glucose conditions.
"Here, we used systematic microscopy-based approaches to compare the NVJ at glucose-replete and -restricted conditions and identified five additional NVJ proteins: the permanent NVJ resident Shr5 and the conditional residents Nsg1, Nsg2, Tcb1 and Pex31."
Falcon deep research report for NSG2
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Falcon supports the interpretation of NSG2 as an INSIG-family ER membrane chaperone/regulator of Hmg2 stability with conditional NVJ localization.
"NSG2/YNL156C is best annotated as an ER and starvation-induced NVJ multi-pass INSIG-family membrane protein that participates in sterol homeostasis primarily by modulating the stability and/or activity of HMG-CoA reductase isozymes."