NCU04637: ProtNLM2 function review

Source method: ProtNLM2. Source version: live API snapshot 2026-09-09T03:00:51.831347+00:00. Accession: Q7S3B9. Raw source: NCU04637-protnlm-source.json.

Exact emitted paragraph

Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis

Atomic claims

Exact claim span Assessment Rationale
Required presynaptically NPI (0) Presynaptic function requires a nervous-system context absent in Neurospora.
at the neuromuscular junction NPI (0) The fungus has no neuromuscular junction. This literal anatomical context is incompatible with the target taxon.
Implicated in synaptic vesicle endocytosis NPI (0) Endocytosis is conserved, but synaptic vesicles specify neuronal trafficking and are not fungal endocytic vesicles.

All literal atoms carry TAXON_CONSTRAINT_VIOLATION. The broader, separately assessed proposition “participates in endocytosis” is CNN (2): current PAINT supports it and characterized fungal Rvs proteins substantiate the transfer. It is not an additional emitted prediction and is excluded from the atomic claim count.

Evidence and provenance

The 467-residue target contains BAR residues 17–269 and SH3 residues 407–467, and PANTHER assigns PTHR47174:SF1, “REDUCED VIABILITY UPON STARVATION PROTEIN 167.” PMID:20610658(https://pubmed.ncbi.nlm.nih.gov/20610658/) explicitly distinguishes Rvs167’s BAR-plus-SH3 architecture from Rvs161, which consists solely of BAR, and demonstrates fungal Rvs complex membrane binding/tubulation. PMID:35976707(https://pubmed.ncbi.nlm.nih.gov/35976707/) studies Rvs-dependent endocytic scission. These findings justify fungal endocytosis, not neuronal anatomy.

The paragraph donor Q8I1C0 is Drosophila pseudoobscura Endophilin-A, whose own text is transferred by similarity from Q8T390. The source phmmer score 39.2 does not justify transferring the fly’s anatomical context. The donor chain is preserved in NCU04637-donor-Q8I1C0.json.

Complete paragraph evidence object

{
  "commentType": "FUNCTION",
  "texts": [
    {
      "evidences": [
        {
          "evidenceCode": "ECO:0008006",
          "id": "ProtNLM2",
          "properties": [
            {
              "key": "model_score",
              "value": "0.39"
            },
            {
              "key": "phmmer_accession",
              "value": "Q8I1C0"
            },
            {
              "key": "phmmer_score",
              "value": "39.2"
            }
          ],
          "source": "Google"
        }
      ],
      "value": "Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis"
    }
  ]
}