This guide records source boundaries and evidence navigation. It is not an independent experiment. Row numbers below are zero-based positions in the preserved 57-assertion review.
PubMed source. Human WDR34 from a HEK293 cDNA library; endogenous association and cellular TAK1 phosphorylation/reporter assays support a context-dependent IL-1/TLR signaling effect. The two named RNAi-3/RNAi-8 plasmids have identical printed target sequences. Sequestration is a discussion model, not purified kinase inhibition.
PubMed source. Human WDR34 cDNA NM_052844.3 and sequence-checked mGFP construct in RPE1 cells; endogenous pericentrosomal and tagged ciliary/cytoplasmic pools. GFP proteolysis may contribute to some diffuse signal. AP-MS and immunoblotting support dynein-2 assembly rather than isolated binary contacts.
PubMed source. Human interaction-screen assertions are independently traced to exact IntAct pairs, PSI-MI assay types, feature sides and ROGIDs. Coilin and TRIM54 are distinct partners. The human TRIM54 ligase-class assertion is IBA evidence, not a new direct enzyme assay.
PubMed source. Human endogenous HEK293 complex association and cell-free translation/pull-down assays support TCTEX1D2 association with WDR34 and WDR60. The WDR34 association is retained as its own positive result; it is not replaced by a different light-chain pair or described as purified affinity measurement.
PubMed source. Selected full-cache methods and literal reciprocal WDR34/WDR60 tandem-affinity records support positive association. The IntAct records are spoke-expanded complex evidence with bait-tag provenance; no direct isolated binding interface is inferred.
PubMed source. Human dynein-2 VIP/reconstitution-in-cell-lysate assays support WDR34 light-chain and heavy-chain assembly interfaces. The explicit knockout experiments in the inspected text concern WDR60/TCTEX1D2, so a WDR34-specific perturbation is not invented for this source. Independent WDR34 experiments establish its retrograde-transport role.
PubMed source. Mechanistic review supplies complex nomenclature and transport context. It is not treated as an independent primary binding or kinetic experiment.
PubMed source. Full main text supports WDR34 interactions with DYNLL2 (80–93) and DYNLRB1 (106–131 region), plus failed rescue by binding-site deletions. Early versus later frameshift clones yield different ciliary phenotypes. Exact supplemental construct tables were unavailable (publisher HTTP403 and PMC timeout); no supplement or figure-pixel reading is claimed.
PubMed source. Independent primary-source reading supports the human dynein-2 architecture and structural interfaces of WDR34. The complete intermediate/light-chain block was removed in the functional construct comparison, not WDR34 alone. The evidence supports contribution to an assembled motor, not autonomous WDR34 ATPase.
PubMed source. The accessible abstract and curated review annotation support dynein-2 context. Full source-specific experiments are not claimed from this secondary overview.
PubMed source. Literal human Q6FHY5–Q96EX3 screen records include validated two-hybrid, array and pooled-selection entries. The unreviewed MEOX2 reference sequence retains the reviewed comparator homeobox. Reference-sequence authentication does not reconstruct a physical screening insert or establish a physiological transcriptional role for DYNC2I2.
PubMed source. Abstract access is supplemented by two literal human WDR60/WDR34 anti-tag co-IP records, one explicitly spoke-expanded. This supports intermediate-chain association while preserving complex-capture and construct limits.
PubMed source. Full article and genuine supplement were inspected. Supplementary Figure 2b lists a positive mouse Wdr34 hit (31 clones) in the DYNLRB2 yeast-two-hybrid screen. This does not establish the imported human male-germ-cell nuclear location; the selected nuclear reagent panel concerns DYNLRB2 and dynein-1 intermediate chains. The source is not described as lacking dynein-2 members.
PubMed source. The original AP-MS network contains the DYNC2I1 bait to DYNC2I2 prey edge 556070, with HPA020607 reagent metadata. The primary graph is distinguished from the derived cell-embedding network; no isolated binary contact is inferred.
PubMed source. Independent full primary reading establishes distinct human WDR34/WDR60 knockout and rescue phenotypes in RPE1 cells. The authors could not obtain reliable quantitative IFT data. DSP-crosslinked immunoprecipitation measures complex association, not binary affinity; the other intermediate chain does not substitute in rescue.
PubMed source. Full accessible primary text supports human fibroblast basal-body/occasional axonemal localization, with mouse MEF/IMCD3/ATDC5 experiments kept separate. The nuclear-membrane staining and antibody limitations are retained as context, not proof of the imported male-germ-cell nucleus.
PubMed source. Genuine full original and normal full cache recovered. A sequence-checked mouse Wdr34 cDNA expressed as GFP fusion in mouse MEFs localizes prominently to cell cortex and filopodia-like protrusions. This is a positive tagged-mouse result, not direct endogenous human filopodial function.
The raw-derived audit preserves reference-sequence ROGIDs and experimental feature sides. It does not claim those reference sequences are the physical assay insert. Positive source records are not rejected solely because their native physiological role is unresolved.
Record: intact:EBI-10439143|imex:IM-23318-24619; psi-mi:"MI:0397"(two hybrid array); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q96EX3 feature sufficient binding region:118-536; participant B uniprotkb:P38432 feature -. Saved record index 23.
Record: intact:EBI-10601828|imex:IM-23318-40342; psi-mi:"MI:1356"(validated two hybrid); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:P38432 feature -; participant B uniprotkb:Q96EX3 feature -. Saved record index 26.
Record: intact:EBI-10285400|imex:IM-23318-10236; psi-mi:"MI:1112"(two hybrid prey pooling approach); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q96EX3 feature sufficient binding region:118-536; participant B uniprotkb:P38432 feature -. Saved record index 27.
Record: intact:EBI-10439157|imex:IM-23318-24620; psi-mi:"MI:0397"(two hybrid array); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q96EX3 feature sufficient binding region:118-536; participant B uniprotkb:Q9BYV2 feature -. Saved record index 24.
Record: intact:EBI-10615756|imex:IM-23318-41698; psi-mi:"MI:1356"(validated two hybrid); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q96EX3 feature -; participant B uniprotkb:Q9BYV2 feature -. Saved record index 25.
Record: intact:EBI-10285414|imex:IM-23318-10237; psi-mi:"MI:1112"(two hybrid prey pooling approach); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q9BYV2 feature -; participant B uniprotkb:Q96EX3 feature sufficient binding region:118-536. Saved record index 28.
Record: intact:EBI-12452851|imex:IM-25054-190; psi-mi:"MI:0676"(tandem affinity purification); psi-mi:"MI:0914"(association); expansion psi-mi:"MI:1060"(spoke expansion); participant A uniprotkb:Q96EX3 feature tandem tag:n-n; participant B uniprotkb:Q8WVS4 feature -. Saved record index 39.
Record: intact:EBI-12452891|imex:IM-25054-192; psi-mi:"MI:0676"(tandem affinity purification); psi-mi:"MI:0914"(association); expansion psi-mi:"MI:1060"(spoke expansion); participant A uniprotkb:Q8WVS4 feature tandem tag:n-n; participant B uniprotkb:Q96EX3 feature -. Saved record index 43.
No matching selected IntAct record was recovered; the actual primary or separate AP-MS evidence is assessed above. This is not negative binding evidence.
No matching selected IntAct record was recovered; the actual primary or separate AP-MS evidence is assessed above. This is not negative binding evidence.
No matching selected IntAct record was recovered; the actual primary or separate AP-MS evidence is assessed above. This is not negative binding evidence.
Record: intact:EBI-24345647|imex:IM-25472-117217; psi-mi:"MI:1356"(validated two hybrid); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q6FHY5 feature -; participant B uniprotkb:Q96EX3 feature sufficient binding region:118-536. Saved record index 20.
Record: intact:EBI-22429055|imex:IM-25472-6460; psi-mi:"MI:1112"(two hybrid prey pooling approach); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q96EX3 feature gal4 dna binding domain:n-n|sufficient binding region:118-536; participant B uniprotkb:Q6FHY5 feature gal4 activation domain:n-n. Saved record index 21.
Record: intact:EBI-22886068|imex:IM-25472-31434; psi-mi:"MI:0397"(two hybrid array); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q96EX3 feature gal4 dna binding domain:n-n|sufficient binding region:118-536; participant B uniprotkb:Q6FHY5 feature gal4 activation domain:n-n. Saved record index 44.
Record: intact:EBI-54798803|imex:IM-29278-17087; psi-mi:"MI:0007"(anti tag coimmunoprecipitation); psi-mi:"MI:0915"(physical association); expansion -; participant A uniprotkb:Q8WVS4 feature tandem tag:c-c; participant B uniprotkb:Q96EX3 feature -. Saved record index 54.
Record: intact:EBI-54949755|imex:IM-29278-23459; psi-mi:"MI:0007"(anti tag coimmunoprecipitation); psi-mi:"MI:0914"(association); expansion psi-mi:"MI:1060"(spoke expansion); participant A uniprotkb:Q8WVS4 feature tandem tag:c-c; participant B uniprotkb:Q96EX3 feature -. Saved record index 65.
No matching selected IntAct record was recovered; the actual primary or separate AP-MS evidence is assessed above. This is not negative binding evidence.
TRIM54/Q9BYV2 has current human GO:0061630 ubiquitin protein ligase activity by IBA (ancestral node PTN007774218); the evidence is explicitly propagated, not a newly reconstructed direct human catalytic assay. Together with the positive literal screen pair it supports binding to an E3 ligase, without implying WDR34 substrate recognition, ubiquitination or catalytic activity.
The literal MEOX2 partner Q6FHY5 is a 303-amino-acid unreviewed record. Comparison with reviewed P50222 (304 aa) gives one insertion outside the conserved homeobox; it does not replace the original participant identity or reconstruct the experimental insert. The verified DNA-binding transcription-factor class supports the binding refinement without a downstream transcriptional claim. Coilin has no comparably established informative binding-class replacement here and remains a positive non-core association.
PANTHER target family contains 651 nodes; the target leaf is PTN002488185. Its exact 536-amino-acid ungapped sequence matches the official target. Five literal IBDs on the inherited path are retained, including node PTN000278083 and the deeper PTN000277823. Neither donor count nor target-self evidence is treated as circular. Current donor records are provenance context, not all independently read primary experiments.
Row 19: GO:0005868 from node PTN000278083; literal descendant evidence MGI:MGI:1919070|UniProtKB:Q96EX3; full original fields in the audit output.
Row 46: GO:0042073 from node PTN000278083; literal descendant evidence UniProtKB:Q580S1|UniProtKB:Q96EX3|ZFIN:ZDB-GENE-070928-9|MGI:MGI:1919070; full original fields in the audit output.
Row 47: GO:0045503 from node PTN000277823; literal descendant evidence MGI:MGI:107743|FB:FBgn0003654|UniProtKB:Q96EX3; full original fields in the audit output.
Row 49: GO:0045504 from node PTN000277823; literal descendant evidence dictyBase:DDB_G0280849; full original fields in the audit output.
Row 52: GO:0097014 from node PTN000278083; literal descendant evidence MGI:MGI:1919070|UniProtKB:H6UI23; full original fields in the audit output.
UniProt rule ARBA00026971: matched condition-set indices 1589. Target FunFam and taxonomic conditions, including exclusions, are evaluated directly in replay.py.
UniProt rule ARBA00027837: matched condition-set indices 24. Target FunFam and taxonomic conditions, including exclusions, are evaluated directly in replay.py.
UniProt rule ARBA00044605: matched condition-set indices 6. Target FunFam and taxonomic conditions, including exclusions, are evaluated directly in replay.py.
UniProt rule ARBA00088183: matched condition-set indices 2. Target FunFam and taxonomic conditions, including exclusions, are evaluated directly in replay.py.
UniProt rule ARBA00092633: matched condition-set indices 1. Target FunFam and taxonomic conditions, including exclusions, are evaluated directly in replay.py.
Human Protein Atlas target page. Per-location metadata: supported centrosome, basal body and cytosol; approved primary cilium; uncertain nucleoplasm, nucleoli and nuclear bodies. Antibody HPA040764 records cover A-431, U-251MG, U2OS, ASC52telo, serum-starved hTERT-RPE1 and RPTEC/TERT1. These are metadata readings, not independent image-pixel interpretations.
R-HSA-5624949: Assembly of the anterograde IFT train. Literal paths: 5624949 → 5624940 → 5624900; 5624949 → 5624945 → 5624940 → 5624900.
R-HSA-5624952: Assembly of the retrograde IFT train. Literal paths: 5624952 → 5625410 → 5625411 → 5625407; 5624952 → 5625425 → 5625410 → 5625411 → 5625407.
R-HSA-5625416: Anterograde IFT. Literal paths: 5625416 → 5624945 → 5624940 → 5624900; 5625416 → 5625417 → 5625411 → 5625407.
R-HSA-5625421: The anterograde IFT train dissociates. Literal paths: 5625421 → 5625417 → 5625411 → 5625407; 5625421 → 5625411 → 5625407.
R-HSA-5625424: The retrograde IFT train dissociates. Literal paths: 5625424 → 5624947 → 5624941 → 5624940 → 5624900; 5625424 → 5624940 → 5624900.
R-HSA-5625426: Retrograde IFT. Literal paths: 5625426 → 5625425 → 5625410 → 5625411 → 5625407; 5625426 → 5624947 → 5624941 → 5624940 → 5624900.
Every target path ends at Q96EX3 residues 1–536 in the cilium (5624900) or ciliary-tip (5625407) compartment. The ciliary-plasm refinement combines the curated cilium pool with the nonmembrane motor identity. Tip states are accepted as part of the principal cycle, not as new measured human kinetics or exclusive residence.
The independent core consultation read the reserved 30320547 and 31451806 bodies. The owner read the other operative sources and performed the raw-data checks. Failed 30649997 supplement routes remain failures; full main-text availability does not imply supplemental construct tables were read. The genuine 36973253 supplement was read at its mouse Wdr34 screen panel, and the nuclear uncertainty is deliberately preserved. Neither prior report availability nor successful workflow status alone establishes biological completion.
The current ubiquitin protein ligase binding definition classifies the bound E3 protein; DNA-binding transcription factor binding likewise classifies the bound transcription factor. Positive literal interactions plus independent partner-class evidence support these refinements without transferring either partner's enzymatic or regulatory activity. The project-specific generic-binding instruction governs the positive coilin row.
For the electronic filopodium row, the positive tagged-mouse observation is retained, but the reported filopodia-like morphology and experimental context do not resolve endogenous human membership in the actin-based compartment. The updated action limits that transfer (PMID:28379358). The direct retrograde-transport support comes from WDR34 binding/rescue and dynein-2 structural experiments, while the older bidirectional excerpt describes the consequences of disturbing the coupled system (PMID:30649997; PMID:31451806; PMID:30320547).