Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:1320004

A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme.

Native Awd NDPK purified from larvae is a hexamer; the Killer of prune P97S enzyme has normal catalytic efficiency but reduced stability.

PMID:14630942

Drosophila awd, the homolog of human nm23, regulates FGF receptor levels and functions synergistically with shi/dynamin during tracheal development.

awd loss causes ectopic tracheal migration and Breathless/FGFR accumulation; awd and shi/dynamin interact genetically.

PMID:19317464

Mapping organelle proteins and protein complexes in Drosophila melanogaster.

PMID:19581292

The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis.

awd loss in follicle cells causes adherens-junction accumulation and epithelial disruption, mediated via Rab5.

PMID:20516215

Drosophila von Hippel-Lindau tumor suppressor gene function in epithelial tubule morphogenesis.

PMID:2175255

A Drosophila gene that is homologous to a mammalian gene associated with tumor metastasis codes for a nucleoside diphosphate kinase.

awd encodes a microtubule-associated NDP kinase; null neuroblasts arrest in metaphase.

PMID:7669763

Mechanism of phosphate transfer by nucleoside diphosphate kinase: X-ray structures of the phosphohistidine intermediate of the enzymes from Drosophila and Dictyostelium.

Crystal structure of phosphorylated Drosophila NDPK shows N-delta phosphohistidine as the sole phosphorylation site.

PMID:8579610

A novel function for nucleoside diphosphate kinase in Drosophila.

Fly NDPK autophosphorylates and phosphorylates ovalbumin in vitro; activity absent in awd mutant extracts.

PMID:8806830

The enzymatic activity of Drosophila AWD/NDP kinase is necessary but not sufficient for its biological function.

An H119A (catalytically dead) awd transgene fails to rescue the null; human nm23-H2 but not nm23-H1 rescues.

PMID:7559441

Point mutations in awdKpn which revert the prune/Killer of prune lethal interaction affect conserved residues that are involved in nucleoside diphosphate kinase substrate binding and catalysis.

Revertants of Kpn lethality are active-site point mutations lacking NDPK activity.

PMID:11343655

Nucleoside diphosphate kinase, a source of GTP, is required for dynamin-dependent synaptic vesicle recycling.

awd mutations enhance shi(ts) synaptic-vesicle internalization defects; NDK enzymatic activity is essential presynaptically.

PMID:24528630

Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23.

In awd mutant follicle and wing-disc cells Notch accumulates in enlarged early endosomes; constitutively active Rab5 does not rescue.

PMID:33019537

The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways.

PMID:11768313

Role of AWD/nucleoside diphosphate kinase in Drosophila development.

PMID:19373545

Developmental function of Nm23/awd: a mediator of endocytosis.

Review summarising awd NDPK activity (H119A abolishes activity and rescue) and endocytic roles; reports Awd-VHL two-hybrid interaction (unpublished).

file:DROME/awd__P08879/awd__P08879-uniprot.txt

UniProt entry P08879 (awd, NDKA_DROME)

PMID:18212059

Awd, the homolog of metastasis suppressor gene Nm23, regulates Drosophila epithelial cell invasion.

Awd is a negative regulator of border cell migration; its down-regulation allows optimal Pvr and JAK/STAT signalling, and its overexpression stalls migration and reverts constitutive Pvr or dominant-negative dynamin phenotypes.

"Here we show that the metastasis suppressor gene homolog Nm23/awd is a negative regulator of border cell migration."

file:DROME/awd__P08879/awd__P08879-deep-research-flybase.md

FlyBase gene description for awd (DROME)