Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Crystal structure and functional analysis identify the P-loop containing protein YFH7 of Saccharomyces cerevisiae as an ATP-dependent kinase.
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YFH7 is an ATP-dependent small-molecule kinase with an as-yet undetermined substrate.
"Structural and ligand binding analysis combined with enzymatic assays suggest that YFH7 is an ATP-dependent small molecule kinase with new substrate specificity."
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YFH7 adopts the PRK/URK/PANK P-loop kinase fold and is yeast-specific.
"Sequence analysis revealed that yeast YFH7 is a yeast-specific protein showing weak similarity with the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop containing kinases."
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A large ~100-residue insertion distinguishes YFH7 from other family members.
"A large insertion of about 100 residues distinguishes YFH7 from other members of the family."
Parallel analysis of tagged deletion mutants efficiently identifies genes involved in endoplasmic reticulum biogenesis.
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Genome-wide deletion screen for genes required for ER (karmellae) biogenesis; the basis for UniProt's hedged "could be involved in endoplasmic reticulum membrane assembly" note for YFH7. The paper is a screen and does not assign YFH7 a molecular mechanism.
"To identify genes required for karmellae assembly, we compared the composition of populations of homozygous diploid S. cerevisiae deletion mutants following 20 generations of growth with and without karmellae."
Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis.
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Quantitative petite-frequency screen linking many deletion strains (including yfh7Δ, source of the AIM12 alias) to increased mitochondrial genome loss; a phenotypic association, not a biochemical function.
"Using computational predictions combined with traditional quantitative experiments, we have identified 100 proteins whose deficiency alters mitochondrial biogenesis and inheritance in Saccharomyces cerevisiae."
YFH7 bioinformatics analysis (P-loop / Walker-A motif scan, PRK/URK/PANK family signature, domain architecture, and taxonomic conservation).
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Canonical Walker A / P-loop motif GSPGSGKS at residues 31-38 and the URK-family diagnostic second block VILEG at ~263 are both retained, indicating an intact small-molecule-kinase catalytic scaffold; the YFH7 protein is Saccharomycetaceae- restricted while the fold is pan-eukaryotic.
"the small-molecule-kinase catalytic scaffold is intact"