Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Requirement of the Lec35 gene for all known classes of monosaccharide-P-dolichol-dependent glycosyltransferase reactions in mammals.
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Lec35p is required for utilization of the mannose donor Dol-P-Man (MPD) in synthesis of both lipid-linked oligosaccharides and glycosylphosphatidylinositols, and is also required for MPD-dependent C-mannosylation and GPD-dependent glucosylation of LLO.
"glucose-P-dolichol (GPD)-dependent glucosylation of LLO. Both were found to require Lec35p"
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Lec35p is not itself the glycosyltransferase; it is not directly required for the enzymatic transfer step and instead controls donor orientation in the ER membrane.
"Lec35p is not directly required for the enzymatic transfer of mannose from the donor to the acceptor substrate"
MPDU1 mutations underlie a novel human congenital disorder of glycosylation, designated type If.
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MPDU1 mutations cause CDG-If; patient fibroblasts accumulate incomplete LLO precursors and normal LLO biosynthesis is restored by expression of wild-type Lec35 cDNA.
"Retroviral-based expression of the normal Lec35 cDNA in primary fibroblasts of patients restored normal lipid-linked oligosaccharide biosynthesis"
Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system.
Defining the membrane proteome of NK cells.
A reference map of the human binary protein interactome.
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HuRI records a binary interaction between MPDU1 (O75352) and MANBAL (Q9NQG1), consistent with the interaction listed in the UniProt entry.
"reference interactome map of human binary protein interactions"
UniProtKB entry O75352 (MPU1_HUMAN)
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UniProt describes MPDU1 as required for normal utilization of Dol-P-Man in synthesis of N-linked and O-linked oligosaccharides and GPI anchors, and as a multi-pass membrane protein of the MPDU1 (TC 2.A.43.3) family; loss of function causes CDG1F.
"Required for normal utilization of mannose-dolichol phosphate"