Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q88KF1 for Pseudomonas putida KT2440 acnB
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UniProt assigns the aconitate-hydratase reaction to Q88KF1.
"Reaction=citrate = D-threo-isocitrate;"
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UniProt assigns a catalytic iron-sulfur cluster.
"Name=[4Fe-4S] cluster;"
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UniProt places Q88KF1 in propanoate degradation.
"PATHWAY: Organic acid metabolism; propanoate degradation."
In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate.
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Purified Salmonella AcnB catalyzes conversion of 2-methyl-cis-aconitate to 2-methylisocitrate.
"homogeneous AcnB protein of S. enterica also had strong aconitase activity and catalyzed the conversion of 2-methyl-cis-aconitate into 2-methylisocitrate"
OpenScientist functional-annotation report for PSEPK acnB
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The report identifies the methylcitrate-cycle AcnB reaction.
"AcnB catalyzes the hydration of **2-methyl-*cis*-aconitate to 2-methylisocitrate** (EC 4.2.1.99)"
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The report records the lack of P. putida-specific RNA-binding evidence.
"Whether *P. putida* AcnB binds specific transcripts, and which ones, has not been established"
Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing
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The methylcitrate cycle is essential for growth on odd-chain fatty acids (propionate, valerate, heptanoate, nonanoate) in P. putida KT2440, and PP_2339 (acnB) is inferred to provide key methylcitrate-cycle dehydratase/hydratase activity; no transposon insertions were recovered in PP_2339, consistent with essentiality during library construction.
Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori
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In Helicobacter pylori, apo-aconitase (after Fe-S cluster loss) binds multiple mRNAs and acts as a posttranscriptional regulator; cited as family-level precedent for possible AcnB moonlighting, but not demonstrated in P. putida.
"electrophoretic mobility shift assays demonstrated that apo-AcnB is able to bind to RNA transcripts of hpn (encoding a nickel-sequestering protein), ahpC (encoding alkyl hydroperoxide reductase), and flgR (encoding flagellum response regulator)"