Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis.
The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature.
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Sulfite reductase defines a newly discovered bottleneck for assimilatory sulfate reduction and is essential for growth and development in Arabidopsis thaliana.
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Reduced SiR activity in sir1-1 caused a 28-fold decrease in sulfur-label incorporation into cysteine, glutathione and protein.
"The 28-fold decrease of
incorporation of (35)S label into Cys, glutathione, and protein in sir1-1 showed
that the decreased activity of SiR generated a severe bottleneck in the
assimilatory sulfate reduction pathway."
Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.
Sulfite reductase protects plants against sulfite toxicity.
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The study identifies sulfite reductase as the enzyme reducing sulfite to sulfide in sulfate assimilation.
"Plant sulfite reductase (SiR; Enzyme Commission 1.8.7.1) catalyzes the reduction
of sulfite to sulfide in the reductive sulfate assimilation pathway."
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Isolation and characterization of a gene for assimilatory sulfite reductase from Arabidopsis thaliana.
C-Terminal Region of Sulfite Reductase Is Important to Localize to Chloroplast Nucleoids in Land Plants.
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AtSiR-YFP distributes mainly in chloroplast stroma and does not preferentially colocalize with DAPI-stained nucleoids.
"The DAPI staining of isolated cps showed that AtSiR-YFP is not preferentially colocalized with cp nucleoids, indicating that AtSiR is mainly localized to the stroma"
DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase.
file:ARATH/SIR/SIR-uniprot.txt
Reversible DNA compaction by sulfite reductase regulates transcriptional activity of chloroplast nucleoids.
Reproducible extraction and interpretation of SIR in PMID28887381 supplements
Structural and mutational studies of an electron transfer complex of maize sulfite reductase and ferredoxin.
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Maize SiR–ferredoxin co-crystals and interface mutants provide comparative plant evidence for Fd-dependent activity; these are not Arabidopsis pairing experiments.
"Mutational analysis of a total of seven basic residues of SiR distributed widely
at the interface of the complex showed their importance for supporting an
efficient Fd-dependent activity and a strong physical binding to Fd."