prom1b notes (Danio rerio, prominin 1 b; UniProt A0A8M2BI60)

2026-09-28 — session log (DANRE_DUPLICATION batch 4, random TGD_tree sample)

Deep research: not available (Edison 402 Payment Required; OpenAI key invalid). Not attempted, per
instructions. Shared literature in ../prom1a/prom1a-notes.md; pair analysis in
../prom1a/prom1a-bioinformatics/RESULTS.md.

Accession A0A8M2BI60 (TrEMBL; RefSeq XP_005170970.1 isoform X8, 867 aa; alias proml2 in UniProt),
ZFIN:ZDB-GENE-031003-1, Ensembl ENSDARG00000034007, chr1. The PANTHER table lists UniProt A0A8M9QBM3 for this
gene; the review uses the accession with GOA rows. Jaszai et al. identify the originally cloned cDNA:
PMID:21407811

Expression

• More robust than prom1a in retina: PMID:21407811
• Adult brain, caudal: PMID:23723983
• Bulk: zygotic only, rising to 26-49 TPM at 3-5 dpf (E-ERAD-475); Bgee calls only retina, brain, larva,
  embryo, bone. Photoreceptor scRNA: rods 6.5 CP10K, cones 1.1-2.1 CP10K, 3-4x prom1a.

Function

• Knockout: PMID:31362982
• Prph2: PMID:31362982
• Allele (full text on PMC, not cached): TALEN 4-bp deletion c.174_177delACCA, p.Pro59Valfs*62; prom1b mRNA reported decreased at 7 dpf in the mutant (the sentence is ambiguous about
  which genotype was compared, so I treat it as indicative only); the authors' antibody did
  not work for immunofluorescence, so Prom1b was not localized in photoreceptors.
• Apical marker: Prom1b-GFP transgene in retinal neuroepithelia (PMID:22492354).

Annotation decisions

• All four rows ACCEPT; photoreceptor outer segment organization (IMP) is the core process.