Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:2145275

An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase.

First identification of the brain B subunit isoform (B2) of V-ATPase; established that brain and kidney B subunit cDNAs represent different isoforms.
The B subunit is one of the two major subunits of the hydrophilic catalytic complex of V-ATPase.

PMID:12643545

Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.

Mass spectrometry detection of V-ATPase B2 (ATP6V1B2) in stage I-IV melanosomes from melanoma cells.

PMID:17897319

Integral and associated lysosomal membrane proteins.

Large-scale proteomics of lysosomal membrane fraction confirms V-ATPase B2 presence at lysosomal membrane.

PMID:19056867

Large-scale proteomics and phosphoproteomics of urinary exosomes.

Mass spectrometry detection of ATP6V1B2 in urinary exosome fraction; likely a contaminant.

PMID:19199708

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

Mass spectrometry detection of ATP6V1B2 in parotid gland exosomes; likely a contaminant.

PMID:22982048

Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.

Used V-ATPase inhibitors to block lysosomal activity; demonstrates V-ATPase dependence of macroautophagy, but not a primary study of ATP6V1B2 function.

PMID:23533145

In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.

Mass spectrometry detection of ATP6V1B2 in urinary exosomes; likely a contaminant.

PMID:25416956

A proteome-scale map of the human interactome network.

High-throughput yeast two-hybrid screen identifies ATP6V1B2 protein interactions; data used for IPI protein binding annotation.

PMID:29993276

H(+)-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney.

B1 subunit localizes to apical membrane domains of thick ascending limb and distal convoluted tubule in human kidney.
B2 subunit is also expressed in TAL, DCT, and CNT in addition to intercalated cells; it colocalizes with B1 in early distal nephron.

PMID:32001091

Structure and Roles of V-type ATPases.

Comprehensive review of V-ATPase structure, function, and disease associations.
V-ATPase is the primary organellar acidification system in eukaryotes.

PMID:32814053

Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.

ATP6V1B2 identified in neurodegenerative disease protein interactome; interacts with HTT, NEFL, and other neurodegeneration-associated proteins.

PMID:33065002

Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.

Complete human V-ATPase cryo-EM structure confirms three copies of B subunit (including B2) in the V1 hexameric ring.
ATP6V1B2 is the non-catalytic B subunit of V1 domain.

PMID:34159380

Nsp2 has the potential to be a drug target revealed by global identification of SARS-CoV-2 Nsp2-interacting proteins.

ATP6V1B2 identified as a SARS-CoV-2 Nsp2-interacting protein; represents a host-pathogen interaction.

Reactome:R-HSA-1222516

Intraphagosomal pH is lowered to 5 by V-ATPase

Reactome:R-HSA-5252133

ATP6AP1 binds V-ATPase

Reactome:R-HSA-74723

Endosome acidification

Reactome:R-HSA-917841

Acidification of Tf:TfR1 containing endosome

Reactome:R-HSA-9639286

RRAGC,D exchanges GTP for GDP

Reactome:R-HSA-9640167

RRAGA,B exchanges GDP for GTP

Reactome:R-HSA-9640168

v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP:SLC38A9:Arginine dissociates yielding v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP and SLC38A9:Arginine

Reactome:R-HSA-9640175

v-ATPase:Ragulator:RagA,B:GDP:RagC,D:GDP binds SLC38A9:Arginine

Reactome:R-HSA-9640195

RRAGA,B hydrolyzes GTP

Reactome:R-HSA-9645598

RRAGC,D hydrolyzes GTP

Reactome:R-HSA-9645608

v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP binds mTORC1

Reactome:R-HSA-9646468

mTORC1 binds RHEB:GTP

Reactome:R-HSA-9858918

MITF-M-dependent ATP6V1B2 gene expression