Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase.
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Recombinant P. aeruginosa KynB has kynurenine formamidase activity; KynB catalyses step 2 of the bacterial kynurenine (anthranilate) pathway.
"This functional prediction was experimentally verified by cloning, expression and enzymatic characterization of recombinant kynurenine formamidase orthologs from Bacillus cereus, Pseudomonas aeruginosa and Ralstonia metallidurans."
Structures of bacterial kynurenine formamidase reveal a crowded binuclear zinc catalytic site primed to generate a potent nucleophile.
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Catalytic properties and structures of three bacterial KynB enzymes, including P. aeruginosa KynB.
"In the present study, we characterize the catalytic properties and present the crystal structures of three bacterial kynurenine formamidases."
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Binuclear zinc catalytic centre.
"The structures reveal a new amidase protein fold, a highly organized and distinctive binuclear Zn2+ catalytic centre"