Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Zebrafish contains two pax6 genes involved in eye development.
Stereospecificity and PAX6 function direct Hoxd4 neural enhancer activity along the antero-posterior axis.
Hsp90 selectively modulates phenotype in vertebrate development.
Subfunctionalization of duplicated zebrafish pax6 genes by cis-regulatory divergence.
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sunrise is a pax6b homeodomain missense (L244P) allele.
"Sequencing of sri homozygotes and heterozygotes identified a leucine to proline missense mutation in a highly conserved residue of the pax6b homeodomain (Figures 1B, S1B, and S1C)."
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Only pax6b is expressed in the pancreas.
"only pax6b is expressed in the pancreas isolated from 6 month old wild type and sri/sri fish, while eyes from the same individuals express both pax6a and pax6b"
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pax6a and pax6b activate reporters at equivalent levels.
"Activation by the two wild type co-orthologues through the P3 homeodomain target [45] (Figure 1D), or the CD19 paired domain target [46] (Figure 1E), was not significantly different, in contrast to the original observations by Nornes et al [43]."
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The pax6b locus lost many ancestral cis-regulatory elements.
"On LG7 the pax6b locus and rcn1 and elp4 exons have been retained, but many of the pax6b control elements, as well as the wt1 homolog, have been lost."
Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors.
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pax6b pancreatic expression is driven by enhancers A and C.
"While both zebrafish pax6 genes are expressed in the developing eye and nervous system, only pax6b is expressed in the endocrine cells of the pancreas."
Pax6a and Pax6b are required at different points in neuronal progenitor cell proliferation during zebrafish photoreceptor regeneration.
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pax6b is required for the first neuronal progenitor division in retinal regeneration.
"Loss of Pax6b expression did not affect Müller glial cell division, but blocked the subsequent first cell division of the neuronal progenitors."
The Pax6b homeodomain is dispensable for pancreatic endocrine cell differentiation in zebrafish.
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pax6b null mutants and morphants lack beta cells and gain epsilon cells.
"Pax6b-depleted embryos have almost no beta cells, a strongly reduced number of delta cells, and a significant increase of epsilon cells."
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The Pax6b homeodomain is needed for lens but not pancreas.
"we show that deletion of the Pax6b homeodomain in zebrafish embryos does not disturb pancreas development, whereas lens formation is strongly affected."
Sequencing of Pax6 loci from the elephant shark reveals a family of Pax6 genes in vertebrate genomes, forged by ancient duplications and divergences.
Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shh in the diencephalon in vertebrates.
Evolution of the vertebrate Pax4/6 class of genes with focus on its novel member, the Pax10 gene.
Molecular description of eye defects in the zebrafish Pax6b mutant, sunrise, reveals a Pax6b-dependent genetic network in the developing anterior chamber.
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pax6b mutants have anterior segment defects.
"We also characterised homozygous pax6b mutants. Mutant embryos have a thick cornea, iris hypoplasia, a shallow anterior chamber and a small lens."
Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade.
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pax6b null mutants have normal habenular markers.
"Homozygous pax6bsa86 mutant embryos display cxcr4b and brn3a expression in the habenulae largely indistinguishable from that of wild type siblings (Fig 2A, 2B, 2E and 2F)."
Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves.
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pax6a/b dosage controls anterior segment development.
"Largely, AS phenotypes of pax6a/b single and double mutants supported an overall correlation between the degree of reduced pax6a/b gene copy number and the severity of ASD (Fig 6B–6I), as observed in mouse studies [11][33]."
Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes.
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pax6b balances endocrine subtypes in pancreas and intestine.
"pax6b loss-of-function does not affect the total number of EECs and PECs but instead disrupts the balance between endocrine cell subtypes, leading to an increase of ghrelin- and motilin-like-expressing cells in both the intestine and pancreas at the expense of other endocrine cells such as beta and delta cells in the pancreas and pyyb-expressing cells in the intestine."
Medaka (Oryzias latipes) Olpax6.2 acquires maternal inheritance and germ cells expression, but functionally degenerate in the eye.