UniProtKB entry Q9W1H8_DROME (acetyl-CoA C-acyltransferase / Mtpbeta, Drosophila melanogaster)
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Mapping organelle proteins and protein complexes in Drosophila melanogaster.
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LOPIT mass-spectrometry organelle-proteomics of Drosophila embryos mapped hundreds of proteins (including Mtpbeta) to subcellular compartments; basis of the HDA mitochondrion annotation.
"we apply LOPIT, a mass-spectrometry based technique that simultaneously maps proteins to specific subcellular compartments, to Drosophila embryos."
Impaired fatty acid oxidation in a Drosophila model of mitochondrial trifunctional protein (MTP) deficiency.
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Key fly paper. Mtpbeta(KO) flies are viable but have reduced lifespan, defective locomotion and reduced fecundity, accumulate acylcarnitine/hydroxyacylcarnitine, and are impaired in long-chain fatty acid beta-oxidation. Basis of the IMP GO:0006635 annotation.
"Our results indicated that both Mtpα(KO) and Mtpβ(KO) flies were impaired in long-chain fatty acid β-oxidation."
An inventory of peroxisomal proteins and pathways in Drosophila melanogaster.
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Proteome-wide bioinformatics inventory of Drosophila peroxisomal/organellar proteins; basis of an ISM (sequence-model) mitochondrion prediction for Mtpbeta. The paper's focus is peroxisomes; the mitochondrion call is a by-product of proteome-wide localization prediction.
"We have analyzed the proteome of Drosophila to identify the proteins involved in peroxisomal biogenesis and homeostasis as well as metabolic enzymes that function within the organelle."