pnut (Peanut, P40797) notes

Septin, SEPT7 class; shared subunit of both Drosophila septin complexes (Sep1-Sep2-Pnut and Sep4-Sep5-Pnut).

Literature journal

• Original identification: pnut required for cytokinesis; mutant imaginal tissues become multinucleate PMID:8181057; localized to cleavage furrow and intercellular bridge PMID:8181057
• Genetic interaction with sina is the only basis for the photoreceptor NAS annotations PMID:8181057 -> marked over-annotated on all septins.
• Embryo-purified complex Pnut/Sep2/Sep1 binds and hydrolyses GTP PMID:8636235; one bound nucleotide per subunit PMID:8636235
• Orc6 binds the Pnut coiled-coil and stimulates complex GTPase PMID:18987337
• Recombinant complex: function depends on GTP domains of Pnut, Sep1 and Sep2 PMID:25355953; Sep2 alone has no GTPase activity PMID:25355953
• Pnut phosphorylation regulates membrane association PMID:29079679
• Septins bundle actin into rings PMID:24633326
• Wound repair: two complexes PMID:38728140; all five septins bind/bundle F-actin individually PMID:38728140; complexes bend bundles PMID:38728140
• Classes PMID:38728140
• Border cell migration PMID:37329886
• Peanut downregulation (miR-8) causes cytokinesis failure in tumour model PMID:26853367
• Apoptosis modifier: pnut mutations suppress RHG killing PMID:15029247
• Parkin ubiquitinates Pnut and Sep1 PMID:17456438

Decisions

• GTPase activity, GTP binding, actin filament binding, septin complex, cleavage furrow, cytokinesis, wound repair: ACCEPT (core).
• protein binding (2x IPI): REMOVE (no informative replacement).
• actin binding (GO:0003779, MMAP screen PMID:11076973): MODIFY to GO:0051015 actin filament binding, which purified septins directly show PMID:38728140. microtubule binding (same screen): KEEP_AS_NON_CORE.
• photoreceptor cell development NAS: MARK_AS_OVER_ANNOTATED.

Deep research (falcon) follow-up

The falcon report describes Pnut as a membrane-associated, polymerising septin scaffold in the Pnut-Sep2-Sep1 hexamer that binds and hydrolyses GTP, with the C-terminal coiled coil required for complex assembly and Orc6 binding, consistent with the accepted core functions. It adds Ran/importin/Anillin-dependent Pnut recruitment to pseudocleavage furrows and a role in planar epithelial cytokinesis and adherens-junction remodelling (Founounou et al. 2013); these papers are not in GOA or the cache, so no NEW annotations were added.