Gene Ontology annotation through association of InterPro records with GO terms

Use of the ND evidence code for Gene Ontology (GO) terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

PMID:21270388

Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability

tam14 is one of 14 novel S. pombe loci named for having transcript levels altered during meiosis (tam1-tam14); this is an expression observation, not a functional determination.

"We refer to the novel protein-coding genes with no apparent induction in meiosis as new1–new25 , and the 14 genes with t ranscripts a ltered in m eiosis as tam1–tam14"
Fourteen novel-gene transcripts, including tam14, were differentially expressed across a synchronized meiotic time course.

"Fourteen transcripts were differentially expressed during meiosis."
tam14 was NOT among the seven genes selected for deletion/functional assessment (tam2, new1, new5, tam7, new8, tam11, new21), so no tam14 deletion phenotype was reported.

"three criteria were used to select seven genes ( tam2 , new1 , new5 , tam7 , new8 , tam11 , and new21 ) for functional assessment"

file:SCHPO/tam14/tam14-deep-research-falcon.md

Falcon deep research report for tam14 (G2TRT3, SPCC330.20)

The functional annotation of tam14 rests on domain/family-level inference from orthologs (RAMP4/SERP1, Ysy6); no deletion, localization, or biochemical study exists for tam14 itself.

"rests primarily on **domain and family-level inference** from well-characterized orthologs"
No targeted deletion, localization, or biochemical study has been published specifically for tam14/SPCC330.20.

"no targeted deletion, localization, or biochemical study has been published specifically for tam14/SPCC330.20"
By family inference tam14 is a small accessory component of the Sec61 translocon at the ER membrane; RAMP4/SERP1 is a tail-anchored ER protein that intercalates the Sec61 lateral gate and contacts translocating nascent chains.

"predicted to function as a **small accessory component of the Sec61 translocon** at the endoplasmic reticulum membrane"
No direct localization data for tam14 in S. pombe cells has been reported.

"No direct localization data for tam14 in *S. pombe* cells has been reported"
The meiotic expression change reflected in the gene's name does not imply a meiosis-specific biochemical function.

"the meiotic expression change alone does not imply a meiosis-specific biochemical function"
S. pombe UPR is mechanistically divergent (no Hac1/XBP1; Ire1-dependent RIDD), so the mammalian ER-stress-induction paradigm for RAMP4/SERP1 may not transfer.

"*S. pombe* lacks Hac1/XBP1 orthologs and instead relies primarily on Ire1-dependent mRNA decay (RIDD)"
Overall functional confidence is moderate at the family level but low for gene-specific detail.

"Moderate for family-level annotation, low for gene-specific detail"