trpC (PA0651, P20577) review notes

Indole-3-glycerol phosphate synthase (IGPS, EC 4.1.1.48; HAMAP MF_00134_B, Pfam IGPS).
In P. aeruginosa TrpC is a monofunctional IGPS (no fused PRAI domain; 278 aa).

Literature (no deep-research provider available; own reading)

Gerth et al. 2012 (abstract only; no PMC full text): purified PaIGPS
characterised PMID:22555873, with the highest turnover
of any characterised IGPS PMID:22555873. A trpC deletion reduced
fitness in synthetic CF sputum PMID:22555873.
The PseudoCAP IMP for GO:0000162 is coded from the deletion; the abstract
reports a fitness defect in sputum medium, not a tryptophan auxotrophy, so the
IMP observation as stated in the abstract is indirect for the process. The
process claim is nevertheless correct: the purified enzyme catalyses a step of
tryptophan biosynthesis, which is direct participation (the enzyme does the
work). The full text may report auxotrophy; not filed as a defect.
PubMed MCP search returned API errors on 2026-11-05.

PseudoCAP IBA-seed assessment

Seed support queried live in QuickGO on 2026-11-05.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN000523336	GO:0004425 indole-3-glycerol-phosphate synthase activity	4	126 (77 taxa)	GO:0004425, IDA, PMID:22555873 (PseudoCAP)	SOUND	Purified enzyme, kcat measured; direct evidence.	E. coli TrpC P00909 (IDA PMID:5332729), M. tuberculosis TrpC P9WFX7 (IDA x2), Arabidopsis IGPS P49572 (EXP)	Yes; three independent experimental seeds
PTN000523336	GO:0000162 L-tryptophan biosynthetic process	3	126 (77 taxa)	GO:0000162, IMP, PMID:22555873 (PseudoCAP)	SOUND	Term correct (the enzyme catalyses step 4/5 of Trp synthesis, shown in the same paper); the abstract's mutant phenotype is a sputum-fitness defect, so IDA from the enzyme assay would be the cleaner basis.	M. tuberculosis TrpC P9WFX7 (IDA x2), E. coli TrpC P00909 (IMP, acts_upstream_of_or_within)	Yes; M. tuberculosis IDA seeds suffice

No PSEUDOCAP or GO_CENTRAL defect. IGPS is a single-reaction enzyme family; both terms suit the node.