GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods.

PMID:31064836

Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism.

HglS catalyzes conversion of 2-oxoadipate to D-2-hydroxyglutarate

"involves the direct conversion of 2OA to d-2HG"
HglS is essential for growth on lysine isomers

"A ΔPP_5260 strain was unable to grow on either isomer of lysine"
HglS is involved in D-lysine catabolic pathway

"PP_5260, showed a significant fitness defect...near other d-lysine catabolic genes in the P. putida genome"
HglS shows high substrate specificity and kinetic parameters

"Kcat values for their given substrates (330 m−1 for PP_5260"
HglS expression is induced by lysine derivatives

"All five d-lysine pathway proteins examined (AmaA [PP_5257], AmaB [PP_5258], PP_4108, YdcJ [PP_5260], and YdiJ [PP_4493[) were upregulated when grown on l-lysine, d-lysine, or 2AA compared to the glucose control"

PMID:32523014

An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism.

HglS crystal structure reveals molecular basis for substrate specificity

"Initially, Hg1S was crystallized without substrate, and the Hg1S structure was solved at 1.1 Å resolution"
Arg74 is critical for substrate specificity

"arginine 74 forms a salt bridge with the distal carboxylate of the 2OA substrate...an R74A mutation abolished enzymatic activity"
HglS is highly specific for 2-oxoadipate substrate

"HglS exhibited activity only with 2OA, and displayed no detectable and statistically significant activity above background"
HglS represents the missing step in plant lysine catabolism

"DUF1338 family proteins catalyze the last unknown step of plant lysine catabolism"