Gene Ontology annotation through association of InterPro records with GO terms.

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods.

file:human/RASA4B/RASA4B-deep-research-openai.md

Deep research on RASA4B function

file:human/RASA4B/RASA4B-deep-research-falcon.md

Falcon deep research on RASA4B function

Annotation ambiguity flagged: an evolutionary genomics analysis (Diez et al., Nucleic Acids Res, 2011, PMID:21447561) reports RASA4B as a truncated duplicate of RASA4 annotated as a pseudogene, whereas UniProt C9J798 and a 2024 platelet proteomics review (O'Donoghue & Smolenski, Biosci Rep, PMID:38808367) treat RASA4B as a distinct protein entry. Direct mechanistic experiments on the RASA4B protein were not retrieved; the existing review's functional assertions rest on family-level inference from RASA4/CAPRI, RASA3, and RASAL1.

"an evolutionary genomics analysis reports that **RASA4B is a truncated duplicate of RASA4 on chromosome 7 and is annotated as a pseudogene**, creating a notable annotation ambiguity between some literature and curated protein databases."
Family-level inference supports a model in which C2/PH module-containing GAP1-family proteins require calcium-dependent membrane recruitment for detectable RasGAP activity, providing mechanistic context for the existing review's Ca2+-regulated translocation model even though direct RASA4B experiments are lacking.

"reviews of tissue homeostasis note that GAP1-family RasGAPs, including RASA4/CAPRI, typically contain N-terminal C2 domains and that RASA4 undergoes calcium-dependent membrane association; soluble RASA4 lacking productive membrane engagement is reported to be devoid of detectable RasGAP activity"