Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Purification and identification of a novel and four known serine proteinase inhibitors secreted by human glioblastoma cells.
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PI15/p25TI was isolated from conditioned medium as a trypsin-binding protein.
"addition, a novel 25-kDa trypsin-binding protein, tentatively named p25TI, was"
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Purified p25TI directly displayed weak trypsin-inhibitor activity.
"p25TI showed weak inhibitory activity against trypsin in reverse"
cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-related proteins, and its frequent expression in human brain cancer cells.
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The cloned PI15 cDNA encodes a 258-amino-acid precursor.
"The cDNA consisted of 1440 nucleotides and encoded a sequence of 258"
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The study proposed signal-peptide, propeptide, and mature-chain organization.
"The deduced structure of P25TI seemed to consist of a putative"
Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity.
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PI15 is recruited into the chlamydial inclusion lumen.
"PI15 was transported into the chlamydial inclusion lumen"
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PI15 binds the CPAF zymogen.
"We show that PI15 binds to the CPAF zymogen"
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Low PI15 concentrations enhance CPAF protease activity.
"we detected enhanced CPAF protease activity in the presence of very low concentrations of rPI15"
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Higher PI15 concentrations inhibit CPAF protease activity.
"increasing concentrations of rPI15 inhibited CPAF protease activity"
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PI15 depletion reduces CPAF activation.
"We observed decreased CPAF activation if PI15 levels were reduced"
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
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The HDA source identified proteins in pooled EPS-urine exosome preparations.
"In pooled EPS-urine exosome samples, ~900 proteins were detected."
Peptidase inhibitor 15 as a novel blood diagnostic marker for cholangiocarcinoma.
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PI15 was frequently detected in cholangiocarcinoma but rarely in hepatocellular carcinoma.
"The positive rate of PI15 expression was 70% in CCA and only 9.1% in"
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Circulating PI15 decreased after cholangiocarcinoma resection.
"Plasma PI15 levels in CCA patients were obviously reduced (p = .0014) after"
UniProtKB record for human PI15 (O43692)
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PI15 is a reviewed 258-amino-acid precursor.
"Reviewed; 258 AA."
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UniProt assigns an N-terminal signal peptide.
"FT SIGNAL 1..19"
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UniProt assigns a propeptide preceding the mature chain.
"FT PROPEP 20..60"
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UniProt assigns an SCP/CAP domain.
"FT DOMAIN 71..211"
Manual literature and annotation review notes for human PI15
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The core direct molecular function is weak serine-protease inhibition.
"Direct reverse-zymography evidence establishes weak inhibition of trypsin"
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The PAINT adaptor route is unsafe despite independent infection-context evidence.
"The PAINT propagation itself is unsafe"
Manual literature synthesis for human PI15 (O43692)
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PI15 is a secreted CAP-family serine-protease inhibitor with a specialized CPAF interaction during infection.
"PI15 is a secreted, processed CAP-family serine-protease inhibitor."