Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.
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P. aeruginosa lipid A carries ester-bound 10:0(3-OH) at positions 3 and 3' of the GlcN disaccharide, matching the C10 specificity of the Pseudomonas LpxA; the analysis is purely chemical and structural, on purified glycolipid.
"3-hydroxydecanoic acid groups [10:0(3-OH)] at positions 3 and 3'"
Expression cloning of a Pseudomonas gene encoding a hydroxydecanoyl-acyl carrier protein-dependent UDP-GlcNAc acyltransferase.
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The P. aeruginosa PAO1 lpxA gene was isolated by expression cloning; the enzyme is a 10-carbon-specific UDP-GlcNAc acyltransferase, >1000-fold selective for 3-hydroxydecanoyl-ACP, and complements an E. coli lpxA temperature-sensitive mutant.
"LpxA assays of extracts of this construct indicated that it is > 1,000-fold more selective for 3-hydroxydecanoyl-ACP than for 3-hydroxymyristoyl-ACP."
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Lipid A made by the complemented strain carries 3-hydroxydecanoate at 3 and 3'.
"consistent with 3-hydroxydecanoate rather than 3-hydroxymyristate at positions 3 and 3'"