file:ORYSI/B8BAB0/B8BAB0-uniprot.txt

UniProtKB TrEMBL entry for B8BAB0, BURP domain-containing protein

B8BAB0 is an unreviewed 623 aa protein (PE 4, predicted) with a SignalP signal peptide (1-21) and a PROSITE BURP domain (384-595), classified in PANTHER PTHR31458:SF2.

file:ORYSI/B8BAB0/B8BAB0-deep-research-falcon.md

Falcon deep research report on B8BAB0 BURP domain-containing protein

Family-level summary of BURP proteins; contains no data on B8BAB0 or OsBURP12 themselves.

file:interpro/panther/PTHR31458/PTHR31458-metadata.yaml

PANTHER family PTHR31458 metadata - Polygalacturonase-associated BURP domain-containing protein

InterPro entry for the family; its description is LLM-generated and unchecked (llm: true, checked: false).

file:ORYSI/B8BAB0/B8BAB0-bioinformatics/RESULTS-rereview.md

B8BAB0 re-review bioinformatics (identity, motif and PANTHER tree placement)

B8BAB0 is 95.6% identical to Q6ZA27 (OsBURP12); it lacks ~31 propeptide residues and has a divergent C-terminal tail that removes the fourth BURP CH motif.
In the PANTHER 19 tree the closest paralog is OsBURP16 (grass duplication PTN000779097); eudicot PG1beta-like proteins join only at the Mesangiosperma node; the PAINT IBD node PTN004827911 (Brassicaceae SF10) does not cover this clade.

PMID:24237159

Overexpression of stress-inducible OsBURP16, the β subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice.

OsBURP16, the nearest paralog of B8BAB0, is a PG1beta-like BURP protein whose overexpression increases PG activity and lowers pectin content.

"Transgenic rice showed enhanced PG activity and reduced pectin content."

PMID:19363683

Genome-wide identification of BURP domain-containing genes in rice reveals a gene family with diverse structures and responses to abiotic stresses.

Defines the 17 rice OsBURP genes and their stress-responsive expression; the cited source for OsBURP12 tissue expression in UniProt Q6ZA27.

PMID:32005373

Ethylene insensitive3-like2 (OsEIL2) confers stress sensitivity by regulating OsBURP16, the β subunit of polygalacturonase (PG1β-like) subfamily gene in rice.

OsEIL2 activates OsBURP14 and OsBURP16 transcription; OsBURP12 is not studied.

PMID:1392611

The beta subunit of tomato fruit polygalacturonase isoenzyme 1: isolation, characterization, and identification of unique structural features.

The tomato PG1 beta subunit (GP1, PTHR31458:SF2) is a cell wall protein that binds the catalytic PG2 and is built from FTNYG-type repeats.

"a cell wall protein that associates with, and apparently regulates, the catalytic PG2 polypeptides"

PMID:26106400

AtPGL3 is an Arabidopsis BURP domain protein that is localized to the cell wall and promotes cell enlargement.

Arabidopsis PGL3 (SF10, the PAINT seed for the Brassicaceae node) localizes to the cell wall and its BURP domain is required to rescue the triple mutant.