Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A proinflammatory cytokine inhibits p53 tumor suppressor activity.
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MIF bypasses p53-mediated growth arrest and apoptosis and suppresses p53 transcriptional activity.
"Treatment with MIF overcame p53 activity in three different biological assays, and suppressed its activity as a transcriptional activator."
Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1.
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Cytosolic MIF binds JAB1/COPS5 and inhibits JAB1-enhanced AP-1 and JNK activity.
"MIF colocalizes with Jab1 in the cytosol, and both endogenous and exogenously added MIF following endocytosis bind Jab1."
Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis.
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Human MIF and Trichinella spiralis MIF catalyze the ketonization of phenylpyruvate and p-hydroxyphenylpyruvate.
"The catalytic specificity of recombinant TsMIF in the ketonization of phenylpyruvate (1.4x10(6) M(-1) x s(-1)) was comparable with that of human MIF, while that of p-hydroxyphenylpyruvate (9.1x10(4) M(-1) x s(-1)) was 71-fold lower."
The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER.
MIF signal transduction initiated by binding to CD74.
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MIF binds the extracellular domain of cell-surface CD74 with nanomolar affinity (Kd ~ 9 nM) to initiate ERK1/2 MAP kinase activation, cell proliferation, and PGE2 production.
"MIF binds to the extracellular domain of CD74, and CD74 is required for MIF-induced activation of the extracellular signal-regulated kinase-1/2 MAP kinase cascade, cell proliferation, and PGE2 production."
Macrophage migration inhibitory factor reduces the growth of virulent Mycobacterium tuberculosis in human macrophages.
Macrophage migration inhibitory factor promotes intestinal tumorigenesis.
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MIF expression and phenylpyruvate tautomerase activity are elevated in human colorectal adenomas.
"MIF expression and tautomerase activity were increased in human and ApcMin/+ mouse intestinal adenomas compared with adjacent normal mucosa."
CD44 is the signaling component of the macrophage migration inhibitory factor-CD74 receptor complex.
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CD44 forms a signaling receptor complex with CD74 that is required for MIF-induced Src and ERK1/2 activation and protection from p53-dependent apoptosis.
"CD74 alone mediated MIF binding; however, MIF-induced ERK1 and ERK2 kinase phosphorylation required the coexpression of full-length CD44."
Alternative chemical modifications reverse the binding orientation of a pharmacophore scaffold in the active site of macrophage migration inhibitory factor.
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Crystal structures of human MIF complexed with carbonyloxime (OXIM) inhibitors characterize the catalytic active site and demonstrate that active-site inhibition abolishes MIF counter-regulation of glucocorticoids.
"We studied the crystal structures of MIF.OXIM-based inhibitors and found two opposite orientations for binding to the active site that were dependent on the chemical structures of an R-group. One orientation was completely unexpected based on previous studies with hydroxyphenylpyruvate and (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester (ISO-1)."
Macrophage migration inhibitory factor (MIF) interacts with Bim and inhibits Bim-mediated apoptosis.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor.
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Cytoplasmic MIF interacts with p115 (USO1) to mediate regulated non-classical secretion of MIF from monocytes and macrophages.
"MIF interacts with p115 in the cytoplasm and the stimulated secretion of MIF results in the accumulation of both proteins in supernatants, which is consistent with MIF release from cells in conjunction with p115."
Dual role of macrophage migration inhibitory factor (MIF) in human breast cancer.
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MIF acts as a chemoattractant and non-cognate ligand for CXCR2 and CXCR4 and promotes breast cancer cell proliferation and invasion.
"Sharing an architectural 3D similarity with the atherogenic and angiogenic chemokine interleukin-8 (IL-8)/CXCL8, MIF was found to function as a non-cognate ligand of CXCR2 and as chemokine-like function (CLF) chemokine. Inflammatory leukocyte recruitment is dependent on MIF-CXCR2 and MIF-CXCR4 interactions [5]."
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Allosteric inhibition of macrophage migration inhibitory factor revealed by ibudilast.
The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF).
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MIF and its homolog DDT bind CD74 with nanomolar affinity to activate CD74-CD44-dependent ERK1/2 MAP kinase signaling and share a conserved N-terminal Pro-2 tautomerase catalytic site.
"We produced recombinant D-DT and demonstrate that it binds with high affinity to CD74, activates the ERK1/2 MAP kinase signaling cascade, and recapitulates many of the inflammatory functions of MIF, including modulation of macrophage migration and glucocorticoid-induced immunosuppression."
Macrophage migration inhibitory factor inhibits the migration of cartilage end plate-derived stem cells by reacting with CD74.
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Recombinant human MIF inhibits migration of cartilage end plate-derived stem cells via CD74.
"Additionally, recombinant human MIF (r-MIF) inhibited the migration of CESCs in a dose-dependent manner. CESCs migration was restored when an antagonist of MIF, (S, R)-3(4-hydroxyphenyl)-4, 5-dihydro-5-isoxazole acetic acid methyl ester (ISO-1), was added."
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
Migration inhibitory factor enhances inflammation via CD74 in cartilage end plates with Modic type 1 changes on MRI.
A proteome-scale map of the human interactome network.
Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.
Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation.
Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
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Proteomic analysis of human saphenous vein extracellular matrix identifies MIF among venous extracellular proteins.
"Extracellular matrix proteins were enriched from venous tissues. The proteomics analysis revealed the presence of >150 extracellular matrix proteins, of which 48 had not been previously detected in venous tissue."
Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor.
Exocytosis of secretory granule lumen proteins
Exocytosis of ficolin-rich granule lumen proteins
Expression of Macrophage migration inhibitory factor